Anticorps Polyclonal de lapin anti-MCT4

• Phare
• Validé par KD/KO

MCT4 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

64

Avis (2)

N° de cat : 22787-1-AP

Synonymes

MCT 4, MCT4, Monocarboxylate transporter 3, Monocarboxylate transporter 4, SLC16A3

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Galerie de données de validation

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  WB analysis of HeLa using 22787-1-AP

  WB result of MCT4 antibody (22787-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCT4 transfected HeLa cells.

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  WB analysis using 22787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22787-1-AP (MCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22787-1-AP (MCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 22787-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22787-1-AP (MCT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 22787-1-AP

  Immunofluorescent analysis of HepG2 cells using 22787-1-AP (MCT4 antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 22787-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 22787-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of HepG2 using 22787-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  FC experiment of HepG2 using 22787-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human MCT4 (22787-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules PC-3, tissu de muscle squelettique de rat
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain, tissu de carcinome à cellules rénales humain, tissu de muscle squelettique de souris, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 49 publications below
• IHC: See 8 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• FC: See 3 publications below

Informations sur le produit

22787-1-AP cible MCT4 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCT4 Protéine recombinante Ag18788
• Nom complet: solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
• Masse moléculaire calculée: 465 aa, 49 kDa
• Poids moléculaire observé: 38-42 kDa
• Numéro d’acquisition GenBank: BC112269
• Symbole du gène: SLC16A3
• Identification du gène (NCBI): 9123
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The monocarboxylate transporter 4 (MCT4, also known as SLC16A3) is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate and ketone bodies. It is widely expressed, particularly strongly in glycolytic tissues such as white skeletal muscle fibres, astrocytes, white blood cells, chondrocytes and some mammalian cell lines. MCT4 is also linked to tumor biology because it mediates lactate transport across membranes resulting in antiapoptotic effects.

Publications

Species	Application	Title
mouse	WB	Nat Cancer; Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer; Authors - Federica Cappellesso
human	WB	Nat Metab; Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma; Authors - Zijian Yang ; KD Validated
		Adv Sci (Weinh); SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle; Authors - Xiaowei She
mouse	IF	Nat Commun; Lactate sensing mechanisms in arterial chemoreceptor cells.; Authors - Hortensia Torres-Torrelo
mouse	WB	Adv Healthc Mater; Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature.; Authors - Zhijie Jiang
human	WB	Proc Natl Acad Sci U S A; Monocarboxylate transporter antagonism reveals metabolic vulnerabilities of viral-driven lymphomas.; Authors - Emmanuela N Bonglack

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Aderonke (Verified Customer) (05-26-2023)

Immunoblotting was done to show the expression of MCT 4 in five head and neck cancer cell lines; only three of the five cell lines demonstrated this. An inhibitor was thereafter used at two differenct concentrations in UM-SCC-17A, and the expression decreased with increasing concentrations.

• Applications: Western Blot
• Primary Antibody Dilution: 1:20,000
• Cell Tissue Type: Head and neck cancer cell line

FH

Juan (Verified Customer) (07-15-2019)

We used the antibody for WB method. The band backgound is clear.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Hela cells

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