Anticorps Polyclonal de lapin anti-MCT2

MCT2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 20355-1-AP

Synonymes

MCT2, SLC16A7

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 20355-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20355-1-AP (MCT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20355-1-AP (MCT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 20355-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20355-1-AP (MCT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 20355-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20355-1-AP (MCT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules HEK-293, cellules K-562
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

20355-1-AP cible MCT2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCT2 Protéine recombinante Ag14201
• Nom complet: solute carrier family 16, member 7 (monocarboxylic acid transporter 2)
• Masse moléculaire calculée: 478 aa, 52 kDa
• Poids moléculaire observé: 80 kDa, 40-52 kDa
• Numéro d’acquisition GenBank: BC030693
• Symbole du gène: SLC16A7
• Identification du gène (NCBI): 9194
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC16A7, also named as MCT2, is a proton-coupled monocarboxylate transporter catalyzing the rapid transport across the plasma membrane of many monocarboxylates. It has been reported that SLC16A7 is the primary monocarboxylate transporter in Purkinje cells and can be detected in brain, cerebellum, liver and kidney, but not in vascular smooth muscle cells, pericytes, endothelium, heart, lung or stomach. There are some isoforms of SLC16A7 with bands around 52 kDa and 40-45 kDa in SDS-PAGE. And the 80 kDa protein might be the dimer which is similar to the papers published. (PMID: 9482213, 15749979, 11291733, 16901736)

Publications

Species	Application	Title
human	WB	Br J Cancer; Autoregulation of H+/lactate efflux prevents monocarboxylate transport (MCT) inhibitors from reducing glycolytic lactic acid production.; Authors - Wiktoria Blaszczak
rat	WB, IF	Anim Cells Syst (Seoul); MCT2 overexpression promotes recovery of cognitive function by increasing mitochondrial biogenesis in a rat model of stroke.; Authors - Xiaorong Yu
human	WB	J Exp Clin Cancer Res; ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer.; Authors - Shuohui Dong
mouse	WB	Biomed Pharmacother; Fibroblast growth factor 21 protects the liver from apoptosis in a type 1 diabetes mouse model via regulating L-lactate homeostasis; Authors - Jiapin Yan