Anticorps Polyclonal de lapin anti-NPT1

NPT1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB

Conjugaison

Non conjugué

Publications

3

N° de cat : 20751-1-AP

Synonymes

Na(+)/PI cotransporter 1, Na/Pi 4, NAPI 1, SLC17A1

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Galerie de données de validation

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  WB analysis of human placenta using 20751-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20751-1-AP (NPT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20751-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20751-1-AP (NPT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

20751-1-AP cible NPT1 dans les applications de WB et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NPT1 Protéine recombinante Ag12819
• Nom complet: solute carrier family 17 (sodium phosphate), member 1
• Masse moléculaire calculée: 467 aa, 51 kDa
• Poids moléculaire observé: 50 kDa, 60-64 kDa
• Numéro d’acquisition GenBank: BC101745
• Symbole du gène: SLC17A1
• Identification du gène (NCBI): 6568
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NPT1 (also known as SLC17A1) is a member of sodium-dependent inorganic phosphate transporters (NPT), which regulates entrance into the cellular membrane. NPT1 is mainly expressed in the kidney transporting small organic anions such as PAH (para-aminohippurate), but it is also found in the liver and brain. NTP1 localizes to the apical membrane of renal proximal tubular cells. NPT1 exits two isoforms due to the alternative splicing. Western blot analysis using this antibody detected a major band around 45-55 kDa in kidney tissue.

Publications

Species	Application	Title
human	WB	Exp Mol Med; Impaired Na+-K+-ATPase signaling in renal proximal tubule contributes to hyperuricemia-induced renal tubular injury.; Authors - Jing Xiao
mouse	WB	Phytomedicine; Eurycomanol alleviates hyperuricemia by promoting uric acid excretion and reducing purine synthesis.; Authors - Ruixia Bao
mouse	WB	Front Pharmacol; Effect of Eurycoma longifolia Stem Extract on Uric Acid Excretion in Hyperuricemia Mice.; Authors - Ruixia Bao