Anticorps Polyclonal de lapin anti-SLC19A1

• Phare
• Validé par KD/KO

SLC19A1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 25958-1-AP

Synonymes

CHMD, FLOT1, Folate transporter 1, FOLT, IFC 1, IFC1, Intestinal folate carrier 1, Placental folate transporter, Reduced folate carrier protein, REFC, RFC, RFC1, SLC19A1

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Galerie de données de validation

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  WB analysis using 25958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25958-1-AP (SLC19A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 25958-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25958-1-AP (SLC19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 25958-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25958-1-AP (SLC19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25958-1-AP (SLC19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25958-1-AP (SLC19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules LNCaP
• Résultats positifs en IHC: tissu placentaire humain, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

25958-1-AP cible SLC19A1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC19A1 Protéine recombinante Ag22921
• Nom complet: solute carrier family 19 (folate transporter), member 1
• Masse moléculaire calculée: 591 aa, 65 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC003068
• Symbole du gène: SLC19A1
• Identification du gène (NCBI): 6573
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC19A1, also named as RFC (reduced folate carrier), is an anionic exchanger that involved in the regulation of intracellular concentrations of folate in exchange for anions. The predicted MW of  SLC19A1 is 65 kDa and 25958-1-AP antibody recognizes the 46 kDa protein which is similar to papers. The 38-40 kDa minor forms and 58 kDa form have also been reported that could be alternate splicing, post-transcriptional modification or the role of accessory proteins. (PMID: 17404734, 9111015)

Publications

Species	Application	Title
mouse	WB	J Clin Invest; An alternatively spliced STING isoform localizes in the cytoplasmic membrane and directly senses extracellular cGAMP; Authors - Xiaobo Li ; KD Validated
human	WB	Cell Chem Biol; A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.; Authors - Yingjie Hou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (06-17-2021)

It does not show a clear band around 46kDa for SLC19A1. It does not work in WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: HepG2, mouse liver and brain

SLC19A1 Antibody Western Blot validation (1:400 dilution) in HepG2, mouse liver and brain (Cat no:25958-1-AP)