Anticorps Polyclonal de lapin anti-EAAT3

• Phare
• Validé par KD/KO

EAAT3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 12686-1-AP

Synonymes

SLC1A1, EAAT 3, Excitatory amino-acid carrier 1, HEAAC1, Slc1 a1

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Galerie de données de validation

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  WB analysis of mouse ovary using 12686-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 12686-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12686-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12686-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12686-1-AP (EAAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12686-1-AP (EAAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12686-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12686-1-AP (EAAT3 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu ovarien de souris, tissu cérébral humain, tissu testiculaire de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

12686-1-AP cible EAAT3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Chèvre, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EAAT3 Protéine recombinante Ag3379
• Nom complet: solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
• Masse moléculaire calculée: 524 aa, 57 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC033040
• Symbole du gène: EAAT3
• Identification du gène (NCBI): 6505
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IHC	Cancer Res; Dysregulated Glutamate Transporter SLC1A1 Propels Cystine Uptake via Xc- for Glutathione Synthesis in Lung Cancer.; Authors - Wenzheng Guo
mouse	WB	Life Sci; Geniposide improves depression-like behavior in prenatal stress male offspring through restoring HPA axis- and glucocorticoid receptor-associated dysfunction; Authors - Yu Ma
mouse	WB	Neuropharmacology; Ginsenoside Rb1 confers neuroprotection via promotion of glutamate transporters in a mouse model of Parkinson's disease.; Authors - Yun-Long Zhang
mouse	WB	J Alzheimers Dis; S-Adenosylmethionine Alleviates Amyloid-β-Induced Neural Injury by Enhancing Trans-Sulfuration Pathway Activity in Astrocytes.; Authors - Xinkun Wan
mouse	WB	Neurosci Lett; Intracellular metabotropic glutamate receptor 5 in spinal dorsal horn neurons contributes to pain in a mouse model of vincristine-induced neuropathic pain; Authors - Xiao Li ; KD Validated
goat	IHC	Anim Sci J; The expression of nutrient chemosensing gate molecules in the ileum and colon is altered for goats fed on a high-grain diet; Authors - Changxin Tian

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