Anticorps Polyclonal de lapin anti-EAAT2
EAAT2 Polyclonal Antibody for WB, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (1)
Applications
WB, IHC, IF, IP, ELISA
Conjugaison
Non conjugué
N° de cat : 22515-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | tissu cérébral de souris, tissu cérébral de rat |
| Résultats positifs en IP | tissu cérébral de souris, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 24 publications below |
| IHC | See 4 publications below |
| IF | See 5 publications below |
Informations sur le produit
22515-1-AP cible EAAT2 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | rat, Humain, porc, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | EAAT2 Protéine recombinante Ag18247 |
| Nom complet | solute carrier family 1 (glial high affinity glutamate transporter), member 2 |
| Masse moléculaire calculée | 574 aa, 62 kDa |
| Poids moléculaire observé | 60-70 kDa, 140 kDa |
| Numéro d’acquisition GenBank | BC132768 |
| Symbole du gène | EAAT2 |
| Identification du gène (NCBI) | 6506 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
EAAT2 (also known as GLT-1), is encoded by SLC1A2 and is a glutamate transporter which can clear the majority of extracellular glutamate in brain and prevent brain seizures.Three isoforms of EAAT2 exist due to the alternative splicing. In addition to the 65-70 kDa monomer form, 130-150 kDa dimer of EAAT2 can also be observed on SDS-PAGE. (PMID: 22114258)
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for EAAT2 antibody 22515-1-AP | Download protocol |
| IP protocol for EAAT2 antibody 22515-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics Hsp90 inhibitor HSP990 in very low dose upregulates EAAT2 and exerts potent antiepileptic activity. | ||
Oxid Med Cell Longev Photobiomodulation Therapy Ameliorates Glutamatergic Dysfunction in Mice with Chronic Unpredictable Mild Stress-Induced Depression. | ||
Aging Cell HIV and drug abuse mediate astrocyte senescence in a β-catenin-dependent manner leading to neuronal toxicity. | ||
Neural Regen Res Astrocyte-neuron communication mediated by the Notch signaling pathway: focusing on glutamate transport and synaptic plasticity | ||
Biochem Pharmacol Regulatory roles of histamine receptor in astrocytic glutamate clearance under conditions of increased glucose variability | ||
Oxid Med Cell Longev Neuroprotective Effect of Ceftriaxone on MPTP-Induced Parkinson's Disease Mouse Model by Regulating Inflammation and Intestinal Microbiota |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Azita (Verified Customer) (05-31-2021) | The cells were subjected to SDS PAGE followed by WB and the EAAT2 antibody was used at 1:1000 dilution and was incubated overnight in 4°C.
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