Anticorps Polyclonal de lapin anti-SLC1A4

SLC1A4 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 13067-2-AP

Synonymes

ASCT 1, ASCT1, SATT, SLC1A4

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Galerie de données de validation

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  WB analysis using 13067-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13067-2-AP (SLC1A4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13067-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13067-2-AP (SLC1A4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 13067-2-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13067-2-AP (SLC1A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13067-2-AP

  IP result of anti-SLC1A4 (IP:13067-2-AP, 4ug; Detection:13067-2-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse kidney using 13067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13067-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules SW480, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique humain, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal de souris, tissu cérébral de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

13067-2-AP cible SLC1A4 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC1A4 Protéine recombinante Ag3763
• Nom complet: solute carrier family 1 (glutamate/neutral amino acid transporter), member 4
• Masse moléculaire calculée: 532 aa, 56 kDa
• Poids moléculaire observé: 62-70 kDa
• Numéro d’acquisition GenBank: BC026216
• Symbole du gène: SLC1A4
• Identification du gène (NCBI): 6509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Cell; Transcriptional regulation by PHGDH drives amyloid pathology in Alzheimer's disease; Authors - Junchen Chen
human	WB	Mol Cancer; CircMYH9 drives colorectal cancer growth by regulating serine metabolism and redox homeostasis in a p53-dependent manner; Authors - Xin Liu
mouse	WB	Ecotoxicol Environ Saf; Toxic effects of maternal cadmium exposure on the metabolism and transport system of amino acids in the maternal livers; Authors - Peng Xu
human	WB	Front Oncol; SLC1A4: A Powerful Prognostic Marker and Promising Therapeutic Target for HCC.; Authors - Xiaozhen Peng
mouse	WB	Res Sq; Generation and characterization of a knock-in mouse model for Spastic Tetraplegia, Thin Corpus Callosum, and Progressive Microcephaly (SPATCCM); Authors - Megan L Ratz
mouse	WB	Mamm Genome; Generation and characterization of a knock-in mouse model for spastic tetraplegia, thin corpus callosum, and progressive microcephaly (SPATCCM); Authors - Megan L Ratz-Mitchem ; KO Validated

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