Anticorps Polyclonal de lapin anti-SLC20A2

• Phare
• Validé par KD/KO

SLC20A2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 12820-1-AP

Synonymes

Pit2, Phosphate transporter 2, hPit2, GLVR-2, GLVR2

---

Galerie de données de validation

• 

  WB analysis using 12820-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12820-1-AP (SLC20A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12820-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 12820-1-AP

  IP result of anti-SLC20A2 (IP:12820-1-AP, 4ug; Detection:12820-1-AP 1:400) with COLO 320 cells lysate 2400ug.

• 

  IHC staining of mouse testis using 12820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 12820-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 12820-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 12820-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SLC20A2 antibody (12820-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules COLO 320, tissu rénal de souris
• Résultats positifs en IP: cellules COLO 320
• Résultats positifs en IHC: human bowen disease, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 1 publications below

Informations sur le produit

12820-1-AP cible SLC20A2 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC20A2 Protéine recombinante Ag3469
• Nom complet: solute carrier family 20 (phosphate transporter), member 2
• Masse moléculaire calculée: 652 aa, 70 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC028600
• Symbole du gène: SLC20A2
• Identification du gène (NCBI): 6575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC20A2 (also known as PiT2) is a sodium (Na)-phosphate (Pi) co-transporter that belongs to the SLC20 family. SLC20A2 is highly expressed in the central nervous system and controls hippocampal-dependent learning and memory (PMID: 38195526). SLC20A2 is the major causative gene for primary familial brain calcification (PFBC), a rare neurological disease characterized by widespread cognitive impairment.

Publications

Species	Application	Title
human	WB	Adv Healthc Mater; Correlating Material Properties to Osteoprotegerin Expression on Nanoparticulate Mineralized Collagen Glycosaminoglycan Scaffolds; Authors - Wei Chen ; KD Validated
mouse	WB	Br J Pharmacol; Hydrogen sulfide inhibits calcification of heart valves; implications for calcific aortic valve disease.; Authors - Katalin Éva Sikura
		Endocrinology; Evocalcet rescues secondary hyperparathyroidism-driven cortical porosity in chronic kidney disease male rats; Authors - Tomoka Hasegawa
mouse	WB	Bone; Growth-related skeletal changes and alterations in phosphate metabolism.; Authors - Toshimi Michigami
human	WB	J Biol Chem; Phosphate (Pi)-regulated heterodimerization of the high-affinity sodium-dependent Pi transporters PiT1/Slc20a1 and PiT2/Slc20a2 underlies extracellular Pi sensing independently of Pi uptake.; Authors - Nina Bon
human	WB	Arterioscler Thromb Vasc Biol; Potential Role of H-Ferritin in Mitigating Valvular Mineralization.; Authors - Katalin Éva Sikura

{{ptg:RelatedPrimaryAntibodies}}