Anticorps Polyclonal de lapin anti-OCTN2

• Phare
• Validé par KD/KO

OCTN2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 16331-1-AP

Synonymes

CDSP, OCTN2, OCTN2VT, SLC22A5

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Galerie de données de validation

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  WB analysis of mouse small intestine using 16331-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 16331-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16331-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 16331-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16331-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 16331-1-AP

  IP Result of anti-OCTN2 (IP:16331-1-AP, 4ug; Detection:16331-1-AP 1:500) with mouse skeletal muscle tissue lysate 2200ug.

• 

  IHC staining of human kidney using 16331-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16331-1-AP (OCTN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16331-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16331-1-AP (OCTN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HEK-293 using 16331-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug OCTN2 antibody (16331-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: tissu d'intestin grêle de souris, tissu de muscle squelettique de souris, tissu pancréatique de souris, tissu placentaire humain, tissu testiculaire de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below

Informations sur le produit

16331-1-AP cible OCTN2 dans les applications de WB, IP, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OCTN2 Protéine recombinante Ag9450
• Nom complet: solute carrier family 22 (organic cation/carnitine transporter), member 5
• Masse moléculaire calculée: 557 aa, 63 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC012325
• Symbole du gène: SLC22A5
• Identification du gène (NCBI): 6584
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OCTN2 (Organic Cation/Carnitine Transporter 2), encoded by SLC22A5, is a ubiquitously expressed organic anion/cation transporter. OCTN2 plays a key role in absorption, tissue distribution and renal reabsorption of L-carnitine which is an essential cofactor in fat metabolism. Defects in the OCTN2 are responsible for primary carnitine deficiency. OCTN2 also transports some important respiratory medicines (such as tiotropium), and due to its expression in the lung, may influence the disposition and absorption of these medicines in the lung.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Global Analysis of Plasma Lipids Identifies Liver-Derived Acylcarnitines as a Fuel Source for Brown Fat Thermogenesis.; Authors - Judith Simcox
rat	WB	Food Funct; Ferulic acid supplementation alleviates hyperuricemia in high-fructose/fat diet-fed rats via promoting uric acid excretion and mediating the gut microbiota; Authors - Nanhai Zhang
mouse	WB	Nutrients; The Enhancement of Acylcarnitine Metabolism by 5-Heptadecylresorcinol in Brown Adipose Tissue Contributes to Improving Glucose and Lipid Levels in Aging Male Mice; Authors - Kuiliang Zhang
rat	WB	Front Pharmacol; L-carnitine prevents lenvatinib-induced muscle toxicity without impairment of the anti-angiogenic efficacy; Authors - Zheng Jing
human	WB	J Neurochem; Identification of drug transporters contributing to oxaliplatin-induced peripheral neuropathy.; Authors - S Fujita ; KD Validated
rat	WB	Toxicol Lett; Lenvatinib causes reduced expression of carnitine/organic cation transporter 2 and carnitine deficiency in the skeletal muscle of rats.; Authors - Zheng Jing