Anticorps Polyclonal de lapin anti-SLC22A7

SLC22A7 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 26796-1-AP

Synonymes

OAT2, NLT, hOAT2

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Galerie de données de validation

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  WB analysis of mouse kidney using 26796-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26796-1-AP (SLC22A7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 26796-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 26796-1-AP (SLC22A7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 26796-1-AP

  IP result of anti-SLC22A7 (IP:26796-1-AP, 4ug; Detection:26796-1-AP 1:1000) with mouse liver tissue lysate 5200ug.

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  IHC staining of human liver using 26796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 26796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 26796-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu hépatique de rat
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

26796-1-AP cible SLC22A7 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC22A7 Protéine recombinante Ag25217
• Nom complet: solute carrier family 22 (organic anion transporter), member 7
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC033805
• Symbole du gène: SLC22A7
• Identification du gène (NCBI): 10864
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Organic anion transporter (OAT)2 (SLC22A7) belongs to the solute carrier group of membrane transport proteins that mediate cellular uptake of numerous organic ions including xenobiotics and endogenous substrates (PMID: 9529348, 20190416). SLC22A7 was originally identified as a novel liver-specific transporter because of its predominant mRNA expression in the rat liver (PMID: 15900017, 25904762). Human SLC22A7 exhibits a robust transport function for a wide array of naturally occurring nucleobases, nucleosides, and nucleotides with a particular role for cGMP (PMID: 18216183).

Publications

Species	Application	Title
human	WB	Biochem Pharmacol; Upregulation of histone acetylation reverses organic anion transporter 2 repression and enhances 5-fluorouracil sensitivity in hepatocellular carcinoma.; Authors - Yingying Wang
mouse	WB	Nutrients; Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent.; Authors - Karolin Weitkunat
human	WB	Drug Metab Pharmacokinet; Inhibiting uptake activity of organic anion transporter 2 by bile acids.; Authors - Yu Wang
mouse	WB	Xenobiotica; Changes in the expression of drug-metabolising enzymes and drug transporters in mice with collagen antibody-induced arthritis; Authors - Chieri Fujino
mouse		Am J Physiol Renal Physiol; Tubular Dysfunction Impairs Renal Excretion of Pseudouridine in Diabetic Kidney Disease; Authors - Anna V Mathew
		Drug Metab Dispos; SND1 Regulates Organic Anion Transporter 2 Protein Expression and Sensitivity of Hepatocellular Carcinoma Cells to 5-Fluorouracil; Authors - Yu Wang

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