Anticorps Polyclonal de lapin anti-SLC24A6

• Phare
• Validé par KD/KO

SLC24A6 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 21430-1-AP

Synonymes

NCKX6, NCLX, SLC24A6, SLC8B1

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Galerie de données de validation

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  WB analysis using 21430-1-AP

  Mouse brain tissue were subjected to SDS PAGE followed by western blot with 21430-1-AP (SLC24A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21430-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21430-1-AP (SLC24A6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21430-1-AP (SLC24A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

21430-1-AP cible SLC24A6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC24A6 Protéine recombinante Ag16034
• Nom complet: solute carrier family 24 (sodium/potassium/calcium exchanger), member 6
• Masse moléculaire calculée: 584 aa, 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC098360
• Symbole du gène: SLC24A6
• Identification du gène (NCBI): 80024
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC24A6, also named as NCLX, is a kind of mitochondrial sodium/calcium antiporter that mediates sodium-dependent calcium efflux from mitochondrion by mediating the exchange of 3 sodium ions per 1 calcium ion. It has been reported that SLC24A6 plays a key role in attenuating potential ROS production and injury upon cardiac reperfusion. SLC24A6 is associated with mitochondrial calcium homeostasis by mediating mitochondrial calcium extrusion. (PMID: 32728214, 20018762, 28219928)

Publications

Species	Application	Title
mouse	WB	Nat Commun; The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury; Authors - Hakjoo Lee
mouse	WB	J Cardiovasc Transl Res; CD137 Signal Mediates Cardiac Ischemia-Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes.; Authors - Yao Xu
human	WB	Oncol Lett; Dihydroartemisinin inhibits liver cancer cell migration and invasion by reducing ATP synthase production through CaMKK2/NCLX; Authors - Jiang Chang
rat	WB,IF	Cell Biochem Biophys; Astragalus Polysaccharides Reduce High-glucose-induced Rat Aortic Endothelial Cell Senescence and Inflammasome Activation by Modulating the Mitochondrial Na+/Ca2+ Exchanger.; Authors - Xin-Yu Miao
rat	WB, IF	J Geriatr Cardiol; The mitochondrial Na(+)/Ca(2+) exchanger may reduce high glucose-induced oxidative stress and nucleotide-binding oligomerization domain receptor 3 inflammasome activation in endothelial cells.; Authors - Yuan Zu ; KD Validated
human	IHC,IF	Cell Death Dis; Lon upregulation contributes to cisplatin resistance by triggering NCLX-mediated mitochondrial Ca2+ release in cancer cells.; Authors - Vidhya Tangeda