Anticorps Polyclonal de lapin anti-SLC25A20

SLC25A20 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 19363-1-AP

Synonymes

CACT, CAC

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Galerie de données de validation

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  WB analysis of mouse heart using 19363-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19363-1-AP (SLC25A20 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19363-1-AP (SLC25A20 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19363-1-AP (SLC25A20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19363-1-AP (SLC25A20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19363-1-AP (SLC25A20 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19363-1-AP (SLC25A20 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19363-1-AP (SLC25A20 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19363-1-AP (SLC25A20 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 19363-1-AP

  Immunofluorescent analysis of Hela cells, using SLC25A20 antibody 19363-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of MCF-7 using 19363-1-AP

  Immunofluorescent analysis of MCF-7 cells, using SLC25A20 antibody 19363-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: Saos-2 cells, tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu testiculaire de rat, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 1 publications below

Informations sur le produit

19363-1-AP cible SLC25A20 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC25A20 Protéine recombinante Ag6896
• Nom complet: solute carrier family 25 (carnitine/acylcarnitine translocase), member 20
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC001689
• Symbole du gène: SLC25A20
• Identification du gène (NCBI): 788
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.; Authors - Jin-Ho Koh
mouse	WB	Commun Biol; Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism; Authors - Michael Landowski
mouse	WB	Nutrients; The Enhancement of Acylcarnitine Metabolism by 5-Heptadecylresorcinol in Brown Adipose Tissue Contributes to Improving Glucose and Lipid Levels in Aging Male Mice; Authors - Kuiliang Zhang
mouse	WB	Sci Rep; Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.; Authors - Lei Wu
mouse	WB	Front Physiol; PPARδ Attenuates Alcohol-Mediated Insulin Resistance by Enhancing Fatty Acid-Induced Mitochondrial Uncoupling and Antioxidant Defense in Skeletal Muscle.; Authors - Jin-Ho Koh
mouse	WB	Cell Rep; Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.; Authors - Nicole M Sayles

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