Anticorps Polyclonal de lapin anti-SLC25A3

• Phare
• Validé par KD/KO

SLC25A3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 10420-1-AP

Synonymes

PiC, PTP, Phosphate transport protein, PHC, OK/SW-cl.48

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Galerie de données de validation

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  WB analysis of HeLa using 10420-1-AP

  WB result of SLC25A3 antibody (10420-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC25A3 transfected HeLa cells.

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  WB analysis using 10420-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10420-1-AP (SLC25A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10420-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10420-1-AP (SLC25A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10420-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10420-1-AP (SLC25A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 10420-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10420-1-AP (SLC25A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10420-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10420-1-AP (SLC25A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules MOLT-4, cellules PC-12
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu hépatique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

10420-1-AP cible SLC25A3 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC25A3 Protéine recombinante Ag0667
• Nom complet: solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40-43 kDa
• Numéro d’acquisition GenBank: BC003504
• Symbole du gène: SLC25A3
• Identification du gène (NCBI): 5250
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mitochondrial phosphate carrier SLC25A3 belongs to the SLC25 family , which is the largest family of solute carriers. SLC25A3 encodes the phosphate carrier PiC that catalyses the import of phosphate into mitochondria where it is needed for ATP synthesis. SLC25A3 has two isoforms SLC25A3-A (a heart-type isoform) and SLC25A3-B (a liver-type isoform) as a result of alternative splicing of exon 3. It has been reported that SLC25A3 mutations are associated with mitochondrial phosphate carrier deficiency, respiratory distress and hypertrophic cardiomyopathy. (PMID: 15984930, 27780865, 20877624)

Publications

Species	Application	Title
human	IF	Am J Respir Cell Mol Biol; Transcriptomics Analysis Identifies the Decline in the AT2 Stem Cell Niche in Aged Human Lungs; Authors - Xue Liu
human	WB	Cell Rep; Mitochondrial transplantation rescues Ca2+ homeostasis imbalance and myocardial hypertrophy in SLC25A3-related hypertrophic cardiomyopathy; Authors - Shuang Li
human	WB,IP,IF	bioRxiv; Endothelial Cu Uptake Transporter CTR1 Senses Disturbed Flow to Promote Atherosclerosis through Cuproptosis; Authors - Varadarajan Sudhahar
mouse	WB	Sci Rep; Activation of PI3K-AKT pathway prevents steroid-induced osteonecrosis of the femoral head via inhibiting Cuproptosis; Authors - Shihua Gao
		Adv Sci (Weinh); HIGD1A Alleviates Oxidative Stress Related Ovarian Hypofunction by Enhancing Granulosa Cell Functions via NF-κB/SOD2 Signaling Pathway; Authors - Huiying Li
human	IHC	Heliyon; Prognostic value and immunological function of cuproptosis-related genes in lung adenocarcinoma; Authors - Liming Zhang

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