Anticorps Polyclonal de lapin anti-SLC25A46

• Phare
• Validé par KD/KO

SLC25A46 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 12277-1-AP

Synonymes

Mitochondrial outer membrane protein SLC25A46, TB1

---

Galerie de données de validation

• 

  WB analysis using 12277-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12277-1-AP (SLC25A46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12277-1-AP

  WB result of SLC25A46 antibody (12277-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC25A46 transfected Jurkat cells.

• 

  WB analysis using 12277-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12277-1-AP (SLC25A46 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 12277-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12277-1-AP (SLC25A46 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IF: See 1 publications below

Informations sur le produit

12277-1-AP cible SLC25A46 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC25A46 Protéine recombinante Ag2923
• Nom complet: solute carrier family 25, member 46
• Masse moléculaire calculée: 418 aa, 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC017169
• Symbole du gène: SLC25A46
• Identification du gène (NCBI): 91137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC25A46 belongs to the SLC25 family of mitochondrial carrier proteins.

Publications

Species	Application	Title
human	WB	Cell Metab; A High-Density Human Mitochondrial Proximity Interaction Network.; Authors - Hana Antonicka
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
human	WB,IF	Brain; Loss of function of SLC25A46 causes lethal congenital pontocerebellar hypoplasia.; Authors - Jijun Wan
human	WB	EMBO Mol Med; SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome.; Authors - Alexandre Janer ; KD Validated
human	WB	J Pers Med; Reanalysis of Exome Data Identifies Novel SLC25A46 Variants Associated with Leigh Syndrome.; Authors - Qifei Li
human	WB	Mol Biol Cell; Rapid degradation of mutant SLC25A46 by the ubiquitin-proteasome system results in MFN1/2 mediated hyperfusion of mitochondria.; Authors - Janos Steffen ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Xianhe (Verified Customer) (07-15-2022)

The WB band is clean and clear, the binding affinity is strong and specific.

• Applications: Western Blot

{{ptg:RelatedPrimaryAntibodies}}