Anticorps Recombinant de lapin anti-SLC25A46

SLC25A46 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240114G3

N° de cat : 83360-2-RR

Synonymes

TB1, Mitochondrial outer membrane protein SLC25A46, 240114G3

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Galerie de données de validation

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  WB analysis using 83360-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83360-2-RR (SLC25A46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of Caco-2 using 83360-2-RR

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SLC25A46 Recombinant antibody (83360-2-RR, Clone:240114G3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using 83360-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SLC25A46 Recombinant antibody (83360-2-RR, Clone:240114G3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83360-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83360-2-RR against Human SLC25A46 were performed. The affinity constant is 0.879 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules MDA-MB-231, tissu cérébral de souris
• Résultats positifs en FC (Intra): cellules Caco-2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83360-2-RR cible SLC25A46 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SLC25A46 Protéine recombinante Ag27293
• Nom complet: solute carrier family 25, member 46
• Masse moléculaire calculée: 418 aa, 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC017169
• Symbole du gène: SLC25A46
• Identification du gène (NCBI): 91137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Solute carrier family 25 member 46 (SLC25A46), a highly conserved protein among various species including vertebrates, is a nuclear gene encoding mitochondrial carriers. SLC25A46 is involved in mitochondrial fission and the transport of lipids from the endoplasmic reticulum to mitochondria (PMID: 33277645). SLC25A46 has 3 isoforms with molecular weights of 30, 37 and 46 kDa, respectively.

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