Anticorps Polyclonal de lapin anti-SLC25A6-Specific

• Phare
• Validé par KD/KO

SLC25A6-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 14841-1-AP

Synonymes

AAC3, ADP,ATP carrier protein 3, ADP/ATP translocase 3, ANT 2, ANT 3, ANT3, ANT3Y, SLC25A6, SLC25A6-Specific

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Galerie de données de validation

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  WB analysis using 14841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14841-1-AP

  WB result of SLC25A6-Specific antibody (14841-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC25A6-Specific transfected HEK-293 cells.

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  WB analysis of human liver using 14841-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14841-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14841-1-AP

  IP Result of anti-SLC25A6-Specific (IP:14841-1-AP, 4ug; Detection:14841-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human skeletal muscle using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skeletal muscle using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 14841-1-AP

  Immunofluorescent analysis of HepG2 cells using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of MCF-7 using 14841-1-AP

  Immunofluorescent analysis of MCF-7 cells using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HEK-293, cellules HEK-293T, tissu cardiaque humain, tissu hépatique humain, tissu placentaire humain
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu cardiaque humain, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14841-1-AP cible SLC25A6-Specific dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: NM_001636
• Symbole du gène: SLC25A6
• Identification du gène (NCBI): 293
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Curr Biol; Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos.; Authors - Ridhima Gomkale
	WB	Hum Mol Genet; Mutations of the mitochondrial carrier translocase channel subunit TIM22 cause early-onset mitochondrial myopathy.; Authors - David Pacheu-Grau
human	WB,IF	Cancer Cell Int; Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer.; Authors - Yi Hao
human	WB	FEBS Lett; TIM29 is a subunit of the human carrier translocase required for protein transport.; Authors - Sylvie Callegari
human	WB,IHC,CoIP	iScience; MRPL12-ANT3 interaction involves in acute kidney injury via regulating MPTP of tubular epithelial cells; Authors - Xingzhao Ji