Anticorps Polyclonal de lapin anti-FATP2

• Phare
• Validé par KD/KO

FATP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

31

N° de cat : 14048-1-AP

Synonymes

ACSVL1, FACVL1, FATP 2, FATP2, Fatty acid transport protein 2, hFACVL1, SLC27A2, THCA CoA ligase, VLACS, VLCS

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Galerie de données de validation

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  WB analysis using 14048-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14048-1-AP (FATP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14048-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14048-1-AP (FATP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14048-1-AP

  IP Result of anti-SLC27A2 (IP:14048-1-AP, 4ug; Detection:14048-1-AP 1:500) with HepG2 cells lysate 3200ug.

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  IHC staining of human kidney using 14048-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14048-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14048-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14048-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14048-1-AP

  Immunofluorescent analysis of HepG2 cells using 14048-1-AP (SLC27A2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, foie de rat, foie de souris, rein de rat, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 28 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

14048-1-AP cible FATP2 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FATP2 Protéine recombinante Ag5217
• Nom complet: solute carrier family 27 (fatty acid transporter), member 2
• Masse moléculaire calculée: 567 aa, 65 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC057770
• Symbole du gène: SLC27A2
• Identification du gène (NCBI): 11001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FATP2 is a member of the FATP family which functions in lipid and bile metabolism. It is a 70-kDa protein predominantly expressed in liver and kidney. Kidney FATP2 is localized exclusively to proximal tubule epithelial cells along the apical but not the basolateral membrane, and regulates lipoapoptosis. FATP2 is involved in metabolism-related diseases including nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM), and is a potential clinical biomarker and therapeutic target.

Publications

Species	Application	Title
mouse	WB	Hepatology; Letter to the editor: Adipose lipolysis is important for ethanol to induce fatty liver in the NIAAA murine model of chronic and binge ethanol feeding; Authors - Wenhao Luo
mouse	WB	Hepatology; Adipose lipolysis is important for ethanol to induce fatty liver in the National Institute on Alcohol Abuse and Alcoholism murine model of chronic and binge ethanol feeding.; Authors - Mallika Mathur
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
mouse	WB	Food Funct; Monounsaturated fatty acid-enriched olive oil exacerbates chronic alcohol-induced hepatic steatosis and liver injury in C57BL/6J mice; Authors - Rui Guo
mouse	WB	Nephrol Dial Transplant; Key enzyme in charge of ketone reabsorption of renal tubular SMCT1 may be a new target in diabetic kidney disease; Authors - Zhenhong Guo