Anticorps Polyclonal de lapin anti-SLC27A3

• Phare
• Validé par KD/KO

SLC27A3 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 12943-1-AP

Synonymes

ACSVL3, FATP 3, FATP3, Fatty acid transport protein 3, SLC27A3, UNQ367/PRO703, VLCS 3

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Galerie de données de validation

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  WB analysis using 12943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12943-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 12943-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12943-1-AP

  IP Result of anti-SLC27A3 (IP:12943-1-AP, 4ug; Detection:12943-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human skin cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 12943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, cellules MCF-7, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu cutané de souris, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

12943-1-AP cible SLC27A3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC27A3 Protéine recombinante Ag4015
• Nom complet: solute carrier family 27 (fatty acid transporter), member 3
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 70-79 kDa
• Numéro d’acquisition GenBank: BC029792
• Symbole du gène: SLC27A3
• Identification du gène (NCBI): 11000
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC27A3, also named as FATP3, is a member of the solute carrier 27A (SLC27A) gene family which encodes fatty acid transport proteins. SLC27A3 is expressed in human neural stem cells and plays a key role in the developmental stage of the central nervous system. It is associated with autism spectrum disorders (ASD) that caused by impaired early brain development.(PMID: 26548558).

Publications

Species	Application	Title
human	WB	Nat Med; PMID: 26950361; Authors - Cholsoon Jang ; KD Validated
mouse	WB	Cancers (Basel); In Vivo Optical Metabolic Imaging of Long-Chain Fatty Acid Uptake in Orthotopic Models of Triple-Negative Breast Cancer.; Authors - Megan C Madonna
mouse	WB	Eur J Pharmacol; A novel therapy for hepatic cholestasis treatment-the combination of rosiglitazone and fenofibrate; Authors - Yuanli Chen
human,mouse	WB,IHC	Front Immunol; Machine-Learning Algorithm-Based Prediction of Diagnostic Gene Biomarkers Related to Immune Infiltration in Patients With Chronic Obstructive Pulmonary Disease.; Authors - Yuepeng Zhang
pig	WB	Mol Reprod Dev; Cloned pig fetuses exhibit fatty acid deficiency from impaired placental transport.; Authors - Zheng Ao
mouse	WB	Int J Mol Sci; Knockdown of VEGFB/VEGFR1 Signaling Promotes White Adipose Tissue Browning and Skeletal Muscle Development; Authors - Mingfa Ling