Anticorps Recombinant de lapin anti-GLUT1

GLUT1 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

6F14

N° de cat : 81463-1-RR

Synonymes

SLC2A1, 6F14, GLUT-1, SLC2A1,GLUT1

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Galerie de données de validation

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  WB analysis using 81463-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81463-1-RR (SLC2A1,GLUT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 81463-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81463-1-RR (GLUT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 81463-1-RR

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 81463-1-RR (GLUT1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293 non bouillies, HeLa cells (incubated at 37°C)
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

81463-1-RR cible GLUT1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GLUT1 Protéine recombinante Ag16282
• Nom complet: solute carrier family 2 (facilitated glucose transporter), member 1
• Masse moléculaire calculée: 492 aa, 54 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BC121804
• Symbole du gène: GLUT1
• Identification du gène (NCBI): 6513
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GLUT1, also known as SLC2A1, is a ubiquitously expressed glucose transporter and is responsible for the basal level of glucose uptake in most cell types. Human erythrocytes express the highest level of GLUT1. Defects in SLC2A1 are the cause of GLUT1 deficiency syndrome type 1 and type 2. High expression of GLUT1 has been reported to be a reliable immunohistochemical marker for juvenile hemangiomas. GLUT1 protein may appear as two or more distinct forms among 43 kDa to 55 kDa due to the different glycosylation states. And the conversion of the highly glycosylated form of GLUT1 to a less glycosylated form has been reported to correlate with differentiation (PMID: 8263524, 23302780).

Publications

Species	Application	Title
human	WB	Br J Cancer; Relationship between the expression of PD-L1 and 18F-FDG uptake in pancreatic ductal adenocarcinoma; Authors - Jiajin Li
mouse,human	WB,IHC	Cell Death Dis; Scutellarin activates IDH1 to exert antitumor effects in hepatocellular carcinoma progression; Authors - Zhao Cui

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