Anticorps Polyclonal de lapin anti-GLUT2

• Phare
• Validé par KD/KO

GLUT2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

63

Avis (5)

N° de cat : 20436-1-AP

Synonymes

Glucose Transporter 2, GLUT 2, GLUT2, SLC2A2

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Galerie de données de validation

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  WB analysis of HEK 293 cells using 20436-1-AP

  WB result of GLUT2 antibody (20436-1-AP, 1:300) with si-Control and si-GLUT2 transfected HEK 293 cells.

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  WB analysis using 20436-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20436-1-AP (GLUT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20436-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20436-1-AP (GLUT2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 20436-1-AP

  IP Result of anti-GLUT2 (IP:20436-1-AP, 4ug; Detection:20436-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of mouse pancreas using 20436-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20436-1-AP (GLUT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 20436-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20436-1-AP (GLUT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HEK 293, cellules HepG2, cellules NIH/3T3, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu pancréatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 49 publications below
• IHC: See 10 publications below
• IF: See 8 publications below

Informations sur le produit

20436-1-AP cible GLUT2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Lasiopodomys Brandtii
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLUT2 Protéine recombinante Ag14204
• Nom complet: solute carrier family 2 (facilitated glucose transporter), member 2
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 60-70 kDa, 38-45 kDa
• Numéro d’acquisition GenBank: BC060041
• Symbole du gène: SLC2A2
• Identification du gène (NCBI): 6514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glucose transporter 2 (GLUT2), also known as solute carrier family 2, facilitated glucose transporter member 2 (SLC2A2), is a transporter protein regulating glucose transport across cell membranes in an insulin-independent manner.

What is the molecular weight of GLUT2? Is GLUT2 post-translationally modified?

GLUT2 can be N-glycosylated. The molecular weight of mature glycosylated GLUT2 transporter is between 55 and 65 kDa, but a minor 38-kDa form is often detected in hepatocytes that may represent a non-glycosylated or proteolytic product (PMID: 12101013).

What is the subcellular localization of GLUT2?

Glucose transporters, including GLUT2, are multiple-pass integral membrane proteins. GLUT2 is present at the plasma membrane but has also been reported to be present at intracellular membranes, including the endoplasmic reticulum (PMID: 12101013).

What molecules can be transported by GLUT2?

Although the main substrate of GLUT2 transport is glucose, it can also transport galactose, mannose, and fructose.

What is the tissue expression pattern of GLUT2?

GLUT2 is expressed in many cell types. High expression of GLUT2 is observed in hepatocytes, where GLUT2 mediates glucose uptake and release regulating glucose levels in blood, in intestinal cells, where it regulates glucose transport at the basolateral membrane and to a lesser extent from the apical membrane, and in the kidney proximal tubule, where it is involved in glucose reabsorption and is present at the basolateral membrane.

Publications

Species	Application	Title
human	WB,IHC	ACS Nano; PMID: 30052036; Authors - Yaoxiang Sun
rat	IHC	Nat Commun; Partial freezing of rat livers extends preservation time by 5-fold; Authors - Shannon N Tessier
human	WB	Clin Transl Med; USP7 promotes non-small-cell lung cancer cell glycolysis and survival by stabilizing and activating c-Abl; Authors - Yuanming He
human,mouse	WB	Cell Mol Gastroenterol Hepatol; Patient-Derived Mutant Forms of NFE2L2/NRF2 Drive Aggressive Murine Hepatoblastomas.; Authors - Huabo Wang
mouse	WB	Mol Metab; T1R2 receptor-mediated glucose sensing in the upper intestine potentiates glucose absorption through activation of local regulatory pathways.; Authors - Kathleen Smith
mouse	IF	Metabolism; Rheb1 promotes glucose-stimulated insulin secretion in human and mouse β-cells by upregulating GLUT expression.; Authors - Yan Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Balawant (Verified Customer) (07-25-2022)

This antibody I used and it is working great. I will recommend this antibody for Immunoblting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Caco2 cell and colon tissue

FH

Susmita (Verified Customer) (04-13-2022)

This antibody is working nicely for my WB

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 1500

FH

Boyan (Verified Customer) (08-24-2021)

Not conclusive. This antibody primarily recognized a band at ~ 90 kd, which did not respond to a Glut2 siRNA treatment. It also weakly recognized a band at ~ 70 kd, which responded to de-glycosylation treatment.

• Applications: Western Blot

FH

Iram (Verified Customer) (09-17-2020)

Excellent GLUT-2 Antibody. Giving a very good results for WB and IF.

• Applications: Western Blot, Flow Cytometry
• Primary Antibody Dilution: 1:2000 for western and 1:200 for IF
• Cell Tissue Type: Human colon cancer

FH

Pradeep (Verified Customer) (09-17-2020)

Very good antibody for western blot. I checked in INS-1 cell line.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: INS-1

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