Anticorps Polyclonal de lapin anti-SLC30A8/ZNT8-Specific

• Phare
• Validé par KD/KO

SLC30A8/ZNT8-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 16169-1-AP

Synonymes

SLC30A8, ZnT 8, Proton-coupled zinc antiporter SLC30A8, ZNT8, ZnT-8

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Galerie de données de validation

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  WB analysis of mouse pancreas using 16169-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16169-1-AP (SLC30A8/ZNT8-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human pancreas using 16169-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: 66198-1-Ig (Insulin antibody).

• 

  IF Staining of HepG2 using 16169-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLC30A8/ZNT8-Specific antibody (16169-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris,
• Résultats positifs en IHC: tissu pancréatique humain, tissu de cancer du foie humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu pancréatique humain,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

16169-1-AP cible SLC30A8/ZNT8-Specific dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: solute carrier family 30 (zinc transporter), member 8
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: NM_173851
• Symbole du gène: SLC30A8
• Identification du gène (NCBI): 169026
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC30A8 (also named as ZNT8) encods the secretory granule zinc transporter, permiting cellular efflux of zinc. SLC30A8 was recently defined as a autoantigen candidates of type 2 diabetes, although the molecular mechanism through which this phenotype is manifest is incompletely understood.

Publications

Species	Application	Title
human	WB	J Biol Chem; Lipid-tuned Zinc Transport Activity of Human ZnT8 Correlates with Risk for Type-2 Diabetes.; Authors - Chengfeng Merriman
rat	WB	J Biol Chem; PMID: 28130446; Authors - Qiong Huang
mouse	WB	J Biol Chem; A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic β-cells.; Authors - Chengfeng Merriman
		J Biol Chem; Highly specific monoclonal antibodies for allosteric inhibition and immunodetection of the human pancreatic zinc transporter ZnT8.; Authors - Chengfeng Merriman
human	WB	J Endocrinol; Lack of ZnT8 protects pancreatic islets from hypoxia- and cytokine induced cell death.; Authors - Maria Karsai
human	IHC	Sci Rep; A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells.; Authors - Shogo Ohta

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