Anticorps Polyclonal de lapin anti-SLC31A1

• Phare
• Validé par KD/KO

SLC31A1 Polyclonal Antibody for WB, IF-P, FC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, FC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 27499-1-AP

Synonymes

COPT1, CTR1, Truncated CTR1 form

---

Galerie de données de validation

• 

  WB analysis of HT-29 using 27499-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 27499-1-AP (SLC31A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27499-1-AP

  WB result of SLC31A1 antibody (27499-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC31A1 transfected HeLa cells.

• 

  WB analysis using 27499-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 27499-1-AP (SLC31A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of human liver cancer using 27499-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SLC31A1 antibody (27499-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 27499-1-AP

  1X10^6 HeLa cells were surface stained with 0.4 ug Anti-Human SLC31A1 (27499-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed .

---

Applications testées

• Résultats positifs en WB: cellules HT-29, cellules Caco-2, cellules HeLa
• Résultats positifs en IF-P: tissu de cancer du foie humain,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

27499-1-AP cible SLC31A1 dans les applications de WB, IHC, IF-P, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC31A1 Protéine recombinante Ag26601
• Nom complet: solute carrier family 31 (copper transporters), member 1
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21-25,30-35,60-70 kDa
• Numéro d’acquisition GenBank: BC013611
• Symbole du gène: SLC31A1
• Identification du gène (NCBI): 1317
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC31A1, also known as CTR1, COPT1 and solute carrier family 31 member 1, is a high-affinity copper transporter found in the cell membrane of human cells. It plays a crucial role in maintaining copper homeostasis within the body and functions as a homotrimer to influence dietary copper absorption in the cell membrane (PMID: 37853210; 28507097). SLC31A1 has three putative transmembrane domains, with an N-terminus located extracellularly and a C-terminus located intracellularly (PMID: 12827356). SLC31A1 can be detected a a 28 kDa band corresponding to glycosylated hCTR1 monomer, and a 24 kDa band that is unglycosylated hCTR1. In some membrane preparations a C-terminal degradation product of 17 kDa is also seen (PMID: 16135512). Additionally, SLC31A1 may also resolve as a 30 to 35 kDa and a 60 to 70 kDa protein on immunoblots, forming oligomers corresponding to the expected size of a homotrimeric complex (PMID: 12827356).

Publications

Species	Application	Title
	WB	Int J Mol Sci; Transcriptional Responses of Copper-Transport-Related Genes ctr1, ctr2 and atox1 and Their Roles in the Regulation of Cu Homeostasis in Yellow Catfish Pelteobagrus fulvidraco; Authors - Hong Yang
human	WB	Cancer Res Commun; Targeting NEDDylation is a Novel Strategy to Attenuate Cisplatin-induced Nephrotoxicity; Authors - Trace M Jones
mouse	IF	Gene; The STAT1-SLC31A1 axis: Potential regulation of cuproptosis in diabetic retinopathy; Authors - Qiang Hu
human	WB,IHC	J Assist Reprod Genet; Blocking lactate regulation of the Grhl2/SLC31A1 axis inhibits trophoblast cuproptosis and preeclampsia development; Authors - Xue-Yan Shen
mouse	IF	Life Sci; Metallothionein rescues doxorubicin cardiomyopathy via mitigation of cuproptosis; Authors - Yan Liu
mouse	WB	J Hazard Mater; Cu(OH)2 nanopesticide induced liver dysfunction in mice by targeting lipoylated tricarboxylic acid cycle proteins via ferredoxin 1; Authors - Zhihua Ren

{{ptg:RelatedPrimaryAntibodies}}