Anticorps Polyclonal de lapin anti-SLC38A1

• Phare
• Validé par KD/KO

SLC38A1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 12039-1-AP

Synonymes

Amino acid transporter A1, ATA1, NAT2, SAT1, SLC38A1, SNAT1

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Galerie de données de validation

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  WB analysis of Jurkat cells using 12039-1-AP

  WB result of SLC38A1 antibody (12039-1-AP, 1:500) with si-Control and si-SLC38A1 transfected Jurkat cells.

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  WB analysis of human brain using 12039-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12039-1-AP (SLC38A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12039-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12039-1-AP (SLC38A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12039-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12039-1-AP (SLC38A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12039-1-AP

  IP Result of anti-SLC38A1 (IP:12039-1-AP, 4ug; Detection:12039-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 12039-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12039-1-AP (SLC38A1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu cérébral humain, tissu rénal humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12039-1-AP cible SLC38A1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC38A1 Protéine recombinante Ag2668
• Nom complet: solute carrier family 38, member 1
• Masse moléculaire calculée: 487 aa, 54 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC010620
• Symbole du gène: SLC38A1
• Identification du gène (NCBI): 81539
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC38A1 (Sodium-coupled neutral amino acid transporter 1), is also named as SNAT1, which is a symporter that cotransports short-chain neutral amino acids and sodium ions from the extraccellular to the intracellular side of the cell membrane (PMID:20599747, PMID:10891391). Inhibition of SLC38A1 reduced tumor growth, cellular migration, invasion, and induced senescence in melanoma cells (PMID:35565278). SLC38A1 was identified as a positive regulator of mTORC1 in neurons, which promoted ischemic brain damage by mTOR-autophagy system (PMID:31552299). SLC38A1 is mainly expressed in brain and placenta.

Publications

Species	Application	Title
human	WB	Nat Commun; C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.; Authors - Dan Liu
human	IF	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
mouse	WB	Environ Pollut; Long-term exposure changes the environmentally relevant bis(2-ethylhexyl) phthalate to be a neuro-hazardous substance disrupting neural homeostasis in emotional and cognitive functions; Authors - Jae Soon Kang
human	WB	J Cell Biol; TFEB controls retromer expression in response to nutrient availability.; Authors - Rachel Curnock
		PLoS One; Promotion of both proliferation and neuronal differentiation in pluripotent P19 cells with stable overexpression of the glutamine transporter slc38a1.; Authors - Ogura Masato M
human	IHC	J Oncol; High Expression Levels of SLC38A1 Are Correlated with Poor Prognosis and Defective Immune Infiltration in Hepatocellular Carcinoma.; Authors - Yun Liu