Anticorps Polyclonal de lapin anti-SLC38A3

• Phare
• Validé par KD/KO

SLC38A3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 14315-1-AP

Synonymes

G17, NAT1, SLC38A3, SN1, SNAT3

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Galerie de données de validation

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  WB analysis using 14315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14315-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 14315-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 14315-1-AP

  IP Result of anti-SLC38A3 (IP:14315-1-AP, 3ug; Detection:14315-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human lung cancer using 14315-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14315-1-AP (SLC38A3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 14315-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14315-1-AP (SLC38A3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 14315-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 14315-1-AP (SLC38A3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 14315-1-AP

  1X10^6 HeLa cells were stained with 0.2ug SLC38A3 antibody (14315-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu hépatique de souris, tissu pancréatique de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14315-1-AP cible SLC38A3 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC38A3 Protéine recombinante Ag5553
• Nom complet: solute carrier family 38, member 3
• Masse moléculaire calculée: 504 aa, 56 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC042875
• Symbole du gène: SLC38A3
• Identification du gène (NCBI): 10991
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC38A3, also known as SNAT3 or SN1, belongs to the SLC38 family of sodium-coupled neutral amino acid transporters. SLC38A3 mediates glutamine transport with sodium and proton coupled manner, and it contributes to ammonia metabolism in the liver and brain. SLC38A3 is highly expressed in the liver and brain astrocytes, with modest expression in the kidney, heart, and muscle. SLC38A3 may migrate higher than the predicted 56 kDa due to the glycosylation.

Publications

Species	Application	Title
mouse	WB	Environ Pollut; Long-term exposure changes the environmentally relevant bis(2-ethylhexyl) phthalate to be a neuro-hazardous substance disrupting neural homeostasis in emotional and cognitive functions; Authors - Jae Soon Kang
mouse	WB,IF,CoIP	Int J Mol Sci; The Role of Nrf2 Transcription Factor and Sp1-Nrf2 Protein Complex in Glutamine Transporter SN1 Regulation in Mouse Cortical Astrocytes Exposed to Ammonia.; Authors - Katarzyna Dąbrowska
mouse	IHC	Cancer Lett; Amino acid transporter SLC38A3 promotes metastasis of non-small cell lung cancer cells by activating PDK1.; Authors - Yanhui Wang
human	WB	Cancer Lett; RCCD1 depletion attenuates TGF-β-induced EMT and cell migration by stabilizing cytoskeletal microtubules in NSCLC cells.; Authors - Junyu Wu
mouse	IF	iScience; Increased NMDARs in neurons and glutamine synthetase in astrocytes underlying autistic-like behaviors of Gabrb1-/- mice; Authors - Jing Wang
mouse	WB	Int J Mol Sci; Sex Modulates Response to Renal-Tubule-Targeted Insulin Receptor Deletion in Mice; Authors - Soha Sohail