Anticorps Recombinant de lapin anti-SLC38A7

SLC38A7 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240147C3

N° de cat : 83346-6-RR

Synonymes

SNAT7, Solute carrier family 38 member 7, Sodium-coupled neutral amino acid transporter 7, FLJ12724, FLJ10815

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Galerie de données de validation

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  WB analysis of Caco-2 using 83346-6-RR

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 83346-6-RR (SLC38A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 83346-6-RR

  human liver tissue were subjected to SDS PAGE followed by western blot with 83346-6-RR (SLC38A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of Caco-2 using 83346-6-RR

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SLC38A7 Recombinant antibody (83346-6-RR, Clone:240147C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of U-251 using 83346-6-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug SLC38A7 Recombinant antibody (83346-6-RR, Clone:240147C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83346-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83346-6-RR against Human SLC38A7 were performed. The affinity constant is 1.31 nM.

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Applications testées

• Résultats positifs en WB: cellules Caco-2, tissu hépatique humain
• Résultats positifs en FC (Intra): cellules Caco-2, cellules U-251

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83346-6-RR cible SLC38A7 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SLC38A7 Protéine recombinante Ag35081
• Nom complet: solute carrier family 38, member 7
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC001961
• Symbole du gène: SLC38A7
• Identification du gène (NCBI): 55238
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC38A7 also known as SNAT7, is a member of the SLC38 family that encodes sodium-coupled neutral amino acid transporters. SLC38A7 is a system N transporter that has a substrate preference for L-glutamine. It also transports other amino acids with polar side chains, as well as L-histidine and L-alanine (PMID: 21511949).

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