Anticorps Polyclonal de lapin anti-ZIP4

• Phare
• Validé par KD/KO

ZIP4 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 20625-1-AP

Synonymes

AEZ, SLC39A4, Zinc transporter ZIP4, ZIP 4, ZIP4, Zrt and Irt like protein 4

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Galerie de données de validation

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  WB analysis of Colo320 cells using 20625-1-AP

  WB result of ZIP4 antibody (20625-1-AP, 1:2000) with si-control and si-ZIP4 transfected Colo320 cells.

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  WB analysis of human jejunum using 20625-1-AP

  human jejunum tissue were subjected to SDS PAGE followed by western blot with 20625-1-AP (ZIP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 20625-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20625-1-AP (ZIP4 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 20625-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20625-1-AP (ZIP4 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse pancreas using 20625-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20625-1-AP (ZIP4 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 20625-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20625-1-AP (ZIP4 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20625-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20625-1-AP (ZIP4 antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Colo320, tissu de jéjunum humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu pancréatique de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 18 publications below
• IHC: See 12 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

20625-1-AP cible ZIP4 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZIP4 Protéine recombinante Ag14524
• Nom complet: solute carrier family 39 (zinc transporter), member 4
• Masse moléculaire calculée: 647 aa, 68 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC062625
• Symbole du gène: SLC39A4
• Identification du gène (NCBI): 55630
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZIP4 is a zinc transporter encoded by the SLC39A4 gene, playing an important role in zinc homeostasis in mammalian cells. Recently, many studies have indicated that ZIP4 plays important roles in many kinds of carcinoma development and progression. ZIP4 may serve as a potential diagnostic and prognostic marker for gliomas and pancreatic cancer.

Publications

Species	Application	Title
human	WB	Gastroenterology; ZIP4 Increases Expression of Transcription Factor ZEB1 to Promote Integrin α3β1 Signaling and Inhibit Expression of the Gemcitabine Transporter ENT1 in Pancreatic Cancer Cells.; Authors - Mingyang Liu
human,mouse	WB,IHC	Gastroenterology; Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2-AKT-TGF-β Signaling Axis in Pancreatic Cancer.; Authors - Xiuhui Shi ; KD Validated
human,mouse	WB,IHC,IF	Clin Cancer Res; ZIP4 Promotes Pancreatic Cancer Progression by Repressing ZO-1 and claudin-1 through a ZEB1-Dependent Transcriptional Mechanism.; Authors - Mingyang Liu ; KD Validated
human,mouse	IHC,WB	Cancer Lett; ZIP4 promotes non-small cell lung cancer metastasis by activating snail-N-cadherin signaling axis.; Authors - Yuanyuan Jiang
human	WB,IF	Int J Mol Sci; Zinc Favors Triple-Negative Breast Cancer's Microenvironment Modulation and Cell Plasticity; Authors - Marina Vogel-González
human	WB,IHC,CoIP	Cancers (Basel); ZIP4 Is a Novel Cancer Stem Cell Marker in High-Grade Serous Ovarian Cancer.; Authors - Qipeng Fan

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