Anticorps Polyclonal de lapin anti-LIV-1/ZIP6

• Phare
• Validé par KD/KO

LIV-1/ZIP6 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 14236-1-AP

Synonymes

Ermelin, LIV 1, LIV1, LIV-1/ZIP6, SLC39A6, Zinc transporter ZIP6, ZIP 6, ZIP6, Zrt and Irt like protein 6

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Galerie de données de validation

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  WB analysis of HeLa using 14236-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14236-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14236-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14236-1-AP

  WB result of LIV-1/ZIP6 antibody (14236-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIV-1/ZIP6 transfected HeLa cells.

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  IHC staining of human pancreas cancer using 14236-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 14236-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules A549, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

14236-1-AP cible LIV-1/ZIP6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LIV-1/ZIP6 Protéine recombinante Ag5478
• Nom complet: solute carrier family 39 (zinc transporter), member 6
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 85-100 kDa, 48 and 32 kDa
• Numéro d’acquisition GenBank: BC039498
• Symbole du gène: SLC39A6
• Identification du gène (NCBI): 25800
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The zinc transporter LIV-1 (also known as SLC39A6 or ZIP6) was originally identified as an estrogen-induced gene in breast cancer cells. Elevated LIV-1 expression is reportedly related to cancer progression in various types of cancer, including breast, prostate, pancreatic, cervical and liver cancers. While correlation of LIV-1 and prognosis may vary. LIV 1 has 3 isoforms with MW of 85-100 (glycosylation and phosphorylation), 48 and 32 kDa while migrates as different bands in different lysates in SDS-PAGE.

Publications

Species	Application	Title
human	WB,IHC	Nat Genet; Genome-wide association study identifies common variants in SLC39A6 associated with length of survival in esophageal squamous-cell carcinoma.; Authors - Wu Chen C ; KD Validated
human	WB	Gastroenterology; Solute Carrier Family 39 Member 6 Gene Promotes Aggressiveness of Esophageal Carcinoma Cells by Increasing Intracellular Levels of Zinc, Activating Phosphatidylinositol 3-Kinase Signaling, and Up-regulating Genes That Regulate Metastasis.; Authors - Xinxin Cheng ; KD Validated
human	WB	Oncotarget; miR-192, a prognostic indicator, targets the SLC39A6/SNAIL pathway to reduce tumor metastasis in human hepatocellular carcinoma.; Authors - Junwei Lian
human	WB,IF	Oncotarget; Co-expression analysis revealed PTCH1-3'UTR promoted cell migration and invasion by activating miR-101-3p/SLC39A6 axis in non-small cell lung cancer: implicating the novel function of PTCH1.; Authors - Xuechao Wan ; KD Validated
human	WB, IHC	J Transl Med; SLC39A6: a potential target for diagnosis and therapy of esophageal carcinoma.; Authors - Xiao-Bin Cui
human	WB,IHC	Oncol Lett; Role of SLC39A6 in the development and progression of liver cancer.; Authors - Zhen Wan ; KD Validated