Anticorps Polyclonal de lapin anti-SLC40A1/FPN1

SLC40A1/FPN1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

50

N° de cat : 26601-1-AP

Synonymes

Ferroportin 1, FPN1, HFE4, IREG1, Iron regulated transporter 1, MST079, MSTP079, MTP1, SLC11A3, SLC40A1

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Galerie de données de validation

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  WB analysis using 26601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26601-1-AP (SLC40A1/FPN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26601-1-AP (SLC40A1/FPN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26601-1-AP (SLC40A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 26601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26601-1-AP (SLC40A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 26601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26601-1-AP (SLC40A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse small intestine using 26601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 26601-1-AP (SLC40A1/FPN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 26601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26601-1-AP (SLC40A1/FPN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HEK-293T, cellules HepG2, cellules SW480, rate de rat, tissu hépatique de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 48 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

26601-1-AP cible SLC40A1/FPN1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC40A1/FPN1 Protéine recombinante Ag24273
• Nom complet: solute carrier family 40 (iron-regulated transporter), member 1
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 62-70 kDa
• Numéro d’acquisition GenBank: BC037733
• Symbole du gène: SLC40A1
• Identification du gène (NCBI): 30061
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC40A1, also named as Ferroportin 1 (FPN1), is an iron transporter which is involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. It has been reported that SLC40A1 mutations may lead to macrophage iron overload, hyperferritinemia, and normal transferrin saturation among ferroportin iron overload patients. This antibody detects 62-70 kDa protein as well as the fragments of 56 kDa and 35 kDa protein similar to papers in SDS-PAGE. (PMID: 21396368,16054062, 18974313)

Publications

Species	Application	Title
human	WB	Redox Biol; NCOA4-mediated ferritinophagy is involved in ionizing radiation-induced ferroptosis of intestinal epithelial cells.; Authors - Hao Zhou
human	WB	Cell Death Dis; Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma; Authors - Weitang Guo
human	WB	Theranostics; Holo-lactoferrin: the link between ferroptosis and radiotherapy in triple-negative breast cancer.; Authors - Zheng Zhang
human	WB	Redox Biol; miR-30-5p-mediated ferroptosis of trophoblasts is implicated in the pathogenesis of preeclampsia.; Authors - Heng Zhang
mouse	WB	Cell Chem Biol; 3D two-photon brain imaging reveals dihydroartemisinin exerts antiepileptic effects by modulating iron homeostasis.; Authors - Chenwen Shao
human	WB	Oxid Med Cell Longev; HO-1 Contributes to Luteolin-Triggered Ferroptosis in Clear Cell Renal Cell Carcinoma via Increasing the Labile Iron Pool and Promoting Lipid Peroxidation.; Authors - Shangting Han