Anticorps Polyclonal de lapin anti-Sodium iodide symporter

Sodium iodide symporter Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

40

Avis (1)

N° de cat : 24324-1-AP

Synonymes

NIS, SLC5A5, Sodium iodide symporter, Sodium/iodide cotransporter

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Galerie de données de validation

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  WB analysis using 24324-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse stomach using 24324-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 24324-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 24324-1-AP

  IP Result of anti-Sodium iodide symporter (IP:24324-1-AP, 4ug; Detection:24324-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human thyroid cancer using 24324-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human thyroid cancer using 24324-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 24324-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 24324-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:50 (under 40x lens).

• 

  FC experiment of MCF-7 using 24324-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug Sodium iodide symporter antibody (24324-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:100.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules SGC-7901, tissu d'estomac de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain, tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 25 publications below
• IHC: See 5 publications below
• IF: See 13 publications below
• IP: See 1 publications below
• FC: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

24324-1-AP cible Sodium iodide symporter dans les applications de WB, IP, IHC, IF, FC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Sodium iodide symporter Protéine recombinante Ag19504
• Nom complet: solute carrier family 5 (sodium iodide symporter), member 5
• Masse moléculaire calculée: 643 aa, 69 kDa
• Poids moléculaire observé: 50-55 kDa, 75-100 kDa
• Numéro d’acquisition GenBank: BC105047
• Symbole du gène: SLC5A5
• Identification du gène (NCBI): 6528
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The sodium iodide symporter (Na+/I - symporter, NIS), encoded by SLC5A5, is an integral plasma membrane glycoprotein that plays an important role in iodide uptake by thyroid cells. Expression of sodium iodide symporter has also been found in extra-thyroidal tissues, including gastric mucosa, lactating mammary gland and salivary glands. Increased expression of sodium iodide symporter has been found in thyroid tissue from patients with Graves' disease as well as papillary thyroid carcinomas. In addition, sodium iodide symporter was found to express in majority of breast cancer tissue but not in normal tissue. Sodium iodide symporter can be a promising diagnostic and therapeutic tool for thyroid cancer and breast cancer. This antibody recognizes the mature approximately 75-100 kDa protein and a partially glycosylated 50-55 kDa protein. (PMID: 12588808, 9525971)

Publications

Species	Application	Title
		Adv Sci (Weinh); Regeneration of Thyroid Glands in the Spleen Restores Homeostasis in Thyroidectomy Mice; Authors - Xue-Jiao Tian
human	WB	Cell Death Differ; REGγ ablation impedes dedifferentiation of anaplastic thyroid carcinoma and accentuates radio-therapeutic response by regulating the Smad7-TGF-β pathway.; Authors - Chan Jiao
human	WB	Cell Death Differ; CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.; Authors - Yina Liao
human	WB	Thyroid; Dimerization of the sodium/iodide symporter (NIS).; Authors - Rebecca J Thompson
human	WB	Cell Chem Biol; Targeting non-canonical pathways as a strategy to modulate the sodium iodide symporter.; Authors - Martin L Read
human	IF	Cancer Lett; IGF2BP2-dependent activation of ERBB2 signaling contributes to acquired resistance to tyrosine kinase inhibitor in differentiation therapy of radioiodine-refractory papillary thyroid cancer.; Authors - Ri Sa

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Katie (Verified Customer) (08-22-2019)

Highly specific antibody for the sodium iodide symporter (NIS) with no background bands in Western blot, used at 1:1000 dilution. Very clear result. Good value for money, and excellent communication prior to delivery receipt.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Thyroid carcinoma cell lines

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