Anticorps Polyclonal de lapin anti-SLC5A8

SLC5A8 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 21433-1-AP

Synonymes

AIT, Apical iodide transporter, SMCT, SMCT1, Sodium iodide-related cotransporter

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Galerie de données de validation

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  WB analysis using 21433-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21433-1-AP (SLC5A8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21433-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21433-1-AP (SLC5A8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 21433-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21433-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 21433-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21433-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 21433-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 21433-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 21433-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 21433-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 21433-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SLC5A8 antibody (21433-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 21433-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SLC5A8 antibody (21433-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu cérébral de souris, tissu cérébral humain, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Informations sur le produit

21433-1-AP cible SLC5A8 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC5A8 Protéine recombinante Ag16041
• Nom complet: solute carrier family 5 (iodide transporter), member 8
• Masse moléculaire calculée: 610 aa, 67 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC110492
• Symbole du gène: SLC5A8
• Identification du gène (NCBI): 160728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The sodium-coupled monocarboxylate transporter 1 (SMCT1; SLC5A8), belonging to the SLC5A family, is a 67-kDa protein that functions as a Na+-coupled electrogenic transporter for SCFAs. SLC5A mRNA and protein are expressed in healthy human, rat, and mouse colon. The protein is localized to the apical membrane of the colonocytes. Immunohistochemical studies revealed the selective localization of SMCT1 on the brush border in the villi of the terminal ileum and crypts of the colon in mice.

Publications

Species	Application	Title
human	IHC,IF	Cell Metab; The probiotic L. casei Zhang slows the progression of acute and chronic kidney disease.; Authors - Han Zhu
rat,human	WB	J Ethnopharmacol; Microbiota-derived short-chain fatty acids mediate the effects of dengzhan shengmai in ameliorating cerebral ischemia via the gut-brain axis; Authors - Hui-Hui Guo
mouse	WB,IHC	Chem Biol Interact; Downregulation of OATP2B1 by proinflammatory cytokines leads to 5-ASA hyposensitivity in Ulcerative colitis; Authors - Ping Li
human	WB	Exp Mol Med; Impaired Na+-K+-ATPase signaling in renal proximal tubule contributes to hyperuricemia-induced renal tubular injury.; Authors - Jing Xiao
human	WB	Front Pharmacol; Ramulus Mori (Sangzhi) Alkaloids (SZ-A) Ameliorate Glucose Metabolism Accompanied by the Modulation of Gut Microbiota and Ileal Inflammatory Damage in Type 2 Diabetic KKAy Mice.; Authors - Quan Liu
mouse	IHC	Sci Rep; Gut-derived butyrate suppresses ocular surface inflammation.; Authors - Laura Schaefer

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