Anticorps Monoclonal anti-SLC6A12

SLC6A12 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2C1G4

Avis (1)

N° de cat : 67700-1-Ig

Synonymes

BGT 1, BGT1, FLJ38727, SLC6A12

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Galerie de données de validation

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  WB analysis using 67700-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67700-1-Ig (SLC6A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67700-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67700-1-Ig (SLC6A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67700-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67700-1-Ig (SLC6A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of Caco-2 using 67700-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed Caco-2 cells using SLC6A12 antibody (67700-1-Ig, Clone: 2C1G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of Caco-2 using 67700-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using SLC6A12 antibody (67700-1-Ig, Clone: 2C1G4 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of H9C2 using 67700-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using SLC6A12 antibody (67700-1-Ig, Clone: 2C1G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 67700-1-Ig

  1x10^6 HepG2 cells were intracellularly stained with 0.2 μg SLC6A12 Monoclonal antibody (67700-1-Ig, Clone:2C1G4) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005)(N/A), or 0.2 μg KLH (66360-2-Ig, Clone: K11A1B2A2) (N/A). Cells were fixed with 4% PFA .

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HepG2, cellules HSC-T6, cellules HuH-7, hépatocytes de souris, tissu hépatique de rat
• Résultats positifs en IF/ICC: cellules Caco-2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

67700-1-Ig cible SLC6A12 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SLC6A12 Protéine recombinante Ag18962
• Nom complet: solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12
• Masse moléculaire calculée: 614 aa, 69 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC126215
• Symbole du gène: SLC6A12
• Identification du gène (NCBI): 6539
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
canine	WB	Am J Physiol Renal Physiol; Mutation of a single threonine in the cytoplasmic NH2 terminus disrupts trafficking of renal betaine-GABA transporter 1 during hypertonic stress.; Authors - Eva S Schweikhard
mouse	WB	Int J Biol Sci; Sodium chloride promotes macrophage pyroptosis and aggravates rheumatoid arthritis by activating SGK1 through GABA receptors Slc6a12; Authors - Xianzheng Zhang
human	WB	Dev Cell; The de novo synthesis of GABA and its gene regulatory function control hepatocellular carcinoma metastasis; Authors - Li Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (06-17-2021)

It works ok. The signal is very weak at 1:2000 dilution. The diluted antibody solution can only be used once.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HepG2, mouse liver and brain

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