Anticorps Polyclonal de lapin anti-Dopamine Transporter/DAT

Dopamine Transporter/DAT Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Conjugaison

Non conjugué

Publications(57)

Avis (1)

N° de cat : 22524-1-AP

Synonymes

2, SLC6A3, DA transporter, DAT, DAT1

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Galerie de données de validation

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  WB analysis using 22524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22524-1-AP (DAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22524-1-AP

  IP result of anti-Dopamine Transporter/DAT (IP:22524-1-AP, 4ug; Detection:22524-1-AP 1:1000) with mouse brain tissue lysate 1720 ug.

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  IHC staining of mouse brain using 22524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 22524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 22524-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DAT antibody (22524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 22524-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Dopamine Transporter/DAT antibody (22524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF-Fro: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 45 publications below
• IHC: See 6 publications below
• IF: See 13 publications below

Informations sur le produit

22524-1-AP cible Dopamine Transporter/DAT dans les applications de WB, IHC, IF-P, IF-Fro, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Dopamine Transporter/DAT Protéine recombinante Ag18297
• Nom complet: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
• Masse moléculaire calculée: 620 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC133003
• Symbole du gène: DAT
• Identification du gène (NCBI): 6531
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DAT, also name as SLC6A3, is dopamine transporter which is a member of the sodium- and chloride- dependent neurotransmitter transporter family. Dopamine(DA) released from neurons is cleared by the DA transporter (DAT). Altered dopaminergic signaling is linked to multiple neuropsychiatric disorders, such as attention deficit hyperactive disorder, mood disorders, schizophrenia, autism and so on (PMID:30755521). 22524-1-AP can detect 70~80kDa band, which is consistent with the report (PMID:12746456).

Publications

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
mouse		Sci Adv; Phosphoglycerate kinase is a central leverage point in Parkinson's disease-driven neuronal metabolic deficits; Authors - Alexandros C Kokotos
mouse	WB	Mov Disord; Fine Particulate Matter Triggers α-Synuclein Fibrillization and Parkinson-like Neurodegeneration; Authors - Xin Yuan
rat	WB	Phytomedicine; Gastrodin relieves Parkinson's disease-related motor deficits by facilitating the MEK-dependent VMAT2 to maintain dopamine homeostasis; Authors - Meihuan Zhao
rat	WB	J Affect Disord; Dysregulation of striatal dopamine D2/D3 receptor-mediated by hypocretin induces depressive behaviors in rats; Authors - Bing Chen
human	WB	Br J Pharmacol; Andrographolide alleviates parkinsonism in MPTP-PD mice via targeting DRP1-mediated mitochondrial fission.; Authors - Ji Geng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (01-20-2025)

Overnight incubation of primary antibody (used at 1:200) at 4 °C followed by 1 h incubation of secondary antibody (Alexa Fluor Donkey anti-rabbit 488, 1:500) at room temperature.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Differentiated SHSY-5Y

Dopamine Transporter/DAT Antibody Immunofluorescence validation (1:200 dilution) in Differentiated SHSY-5Y (Cat no:22524-1-AP)

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