Anticorps Polyclonal de lapin anti-CAT-1

• Phare
• Validé par KD/KO

CAT-1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 14195-1-AP

Synonymes

Amino acid transporter cationic 1, ATRC1, CAT 1, CAT1, CAT-1, HCAT1, REC1L, SLC7A1

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Galerie de données de validation

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  WB analysis of rat brain/liver tissue using 14195-1-AP

  WB result of 14195-1-AP from Dilwar in Rat tissue. Rat brain (a), heart (b) liver (c) mitochondria and liver homogenate (d), (40 ug with 10% PAGE) probed with 1:1000 dilution.

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  WB analysis using 14195-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14195-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14195-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14195-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14195-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human osteosarcoma using 14195-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14195-1-AP (CAT-1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human osteosarcoma using 14195-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14195-1-AP (CAT-1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HepG2, cellules Jurkat, tissu cardiaque de souris, tissu cérébral/hépatique de rat, tissu hépatique de rat
• Résultats positifs en IHC: tissu d'ostéosarcome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

14195-1-AP cible CAT-1 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAT-1 Protéine recombinante Ag5398
• Nom complet: solute carrier family 7 (cationic amino acid transporter, y+ system), member 1
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 67-70 kDa
• Numéro d’acquisition GenBank: BC063303
• Symbole du gène: SLC7A1
• Identification du gène (NCBI): 6541
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Cell; In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.; Authors - Hongling Huang ; KO Validated
mouse	IHC	J Cachexia Sarcopenia Muscle; Derangements of amino acids in cachectic skeletal muscle are caused by mitochondrial dysfunction.; Authors - Thomas Kunzke
mouse, human	WB	J Steroid Biochem Mol Biol; High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1.; Authors - Rui Wang
human	WB	Mol Cell Proteomics; Hypoxia is a dominant remodeler of the effector T cell surface proteome relative to activation and regulatory T cell suppression.; Authors - James R Byrnes
human	IHC	Front Oncol; An Inflammatory Response-Related Gene Signature Can Impact the Immune Status and Predict the Prognosis of Hepatocellular Carcinoma.; Authors - Zhuo Lin
rat	IHC	Exp Neurol; Pseudotype-dependent lentiviral transduction of astrocytes or neurons in the rat substantia nigra.; Authors - Cannon Jason R JR