Anticorps Recombinant de lapin anti-SLC7A11/xCT

SLC7A11/xCT Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

230227C5

N° de cat : 82115-2-RR

Synonymes

SLC7A11, xCT, 230227C5, Amino acid transport system xc, Amino acid transport system xc-

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Galerie de données de validation

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  IF Staining of A549 using 82115-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SLC7A11 antibody (82115-2-RR, Clone: 230227C5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of A549 using 82115-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SLC7A11 antibody (82115-2-RR, Clone: 230227C5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 82115-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human SLC7A11 (82115-2-RR, Clone:230227C5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A549 using 82115-2-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Anti-Human SLC7A11 (82115-2-RR, Clone:230227C5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules A549,
• Résultats positifs en FC (Intra): cellules A549, cellules HeLa

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

82115-2-RR cible SLC7A11/xCT dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SLC7A11/xCT Protéine recombinante Ag25431
• Nom complet: solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
• Masse moléculaire calculée: 55 kDa
• Numéro d’acquisition GenBank: BC012087
• Symbole du gène: SLC7A11
• Identification du gène (NCBI): 23657
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC7A11 (also known as xCT) is a membrane transporter involved in the uptake of cystine. It promotes glutathione synthesis through the uptake of cystine and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers including lung cancer.

Publications

Species	Application	Title
human	WB	Front Immunol; A disulfidptosis-related lncRNA signature for analyzing tumor microenvironment and clinical prognosis in hepatocellular carcinoma; Authors - Haishui Zheng
human	WB	Biofactors; Induction of UBQLN1-mediated PGC1α stability by isoliensinine overcame hypoxia-induced resistance in liver cancer cells; Authors - Xuefen Xu
mouse	IF	Ren Fail; Dapagliflozin improves diabetic kidney disease by inhibiting ferroptosis through β-hydroxybutyrate production; Authors - Yan Tian

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