Anticorps Recombinant de lapin anti-SLC7A11/xCT

SLC7A11/xCT Recombinant Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230227C5

N° de cat : CL488-82115-2

Synonymes

SLC7A11, xCT, 230227C5, Amino acid transport system xc, Amino acid transport system xc-

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Galerie de données de validation

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  IF Staining of HeLa using CL488-82115-2

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 SLC7A11/xCT antibody (CL488-82115-2, Clone: 230227C5 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-82115-2

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Slc7a11/Xct Recombinant Antibody (CL488-82115-2, Clone:230227C5)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A549 using CL488-82115-2

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Slc7a11/Xct Recombinant Antibody (CL488-82115-2, Clone:230227C5)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa, cellules A549

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-82115-2 cible SLC7A11/xCT dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SLC7A11/xCT Protéine recombinante Ag25431
• Nom complet: solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
• Masse moléculaire calculée: 55 kDa
• Numéro d’acquisition GenBank: BC012087
• Symbole du gène: SLC7A11
• Identification du gène (NCBI): 23657
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC7A11 (also known as xCT) is a membrane transporter involved in the uptake of cystine. It promotes glutathione synthesis through the uptake of cystine and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers including lung cancer.

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