Anticorps Polyclonal de lapin anti-SLC7A6

SLC7A6 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 13823-1-AP

Synonymes

KIAA0245, LAT 2, LAT3, SLC7A6, Y+L amino acid transporter 2, y+LAT 2, Y+LAT2

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Galerie de données de validation

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  WB analysis using 13823-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13823-1-AP (SLC7A6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 13823-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13823-1-AP (SLC7A6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 13823-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13823-1-AP (SLC7A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13823-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13823-1-AP (SLC7A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13823-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13823-1-AP (SLC7A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13823-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13823-1-AP (SLC7A6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

13823-1-AP cible SLC7A6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poulet
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC7A6 Protéine recombinante Ag4924
• Nom complet: solute carrier family 7 (cationic amino acid transporter, y+ system), member 6
• Masse moléculaire calculée: 515 aa, 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC028216
• Symbole du gène: SLC7A6
• Identification du gène (NCBI): 9057
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
chicken	WB	Front Physiol; Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers; Authors - Yun Hu
chicken	WB	J Anim Sci; Zinc proteinate with moderate chelation strength enhances zinc absorption by upregulating the expression of zinc and amino acid transporters in primary cultured duodenal epithelial cells of broiler embryos; Authors - Yun Hu
chicken	WB	Poult Sci; The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers; Authors - Yun Hu
human	WB,IHC	Neoplasia; RUNX2 enhances bladder cancer progression by promoting glutamine metabolism; Authors - Zhigang Huang
		Haematologica; RNA helicase DHX15 exemplifies a unique dependency in acute leukemia; Authors - Hao Guo

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