Anticorps Polyclonal de lapin anti-SLD5

SLD5 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 13315-1-AP

Synonymes

GINS4, GINS complex subunit 4, DNA replication complex GINS protein SLD5, N-terminally processed

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Galerie de données de validation

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  WB analysis of mouse thymus using 13315-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13315-1-AP (SLD5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13315-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13315-1-AP (SLD5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 13315-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SLD5 antibody (13315-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu de thymus de souris, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13315-1-AP cible SLD5 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLD5 Protéine recombinante Ag4158
• Nom complet: GINS complex subunit 4 (Sld5 homolog)
• Masse moléculaire calculée: 223 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC027454
• Symbole du gène: SLD5
• Identification du gène (NCBI): 84296
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLD5 (DNA replication complex GINS protein SLD5) is also named as GINS4 (GINS complex subunit 4). SLD5 is required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PMID: 17417653). SLD5 forms a GINS complex with PSF1, PSF2 and PSF3, which is essential for the initiation of DNA replication in lower eukaryotes (PMID: 24244394). SLD5 is a member of the GINS complex, essential for DNA replication in eukaryotes. It has been reported that SLD5 is involved in early embryogenesis in the mouse, and cell cycle progression and genome integrity in Drosophila (PMID: 27499248).

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