Anticorps Polyclonal de lapin anti-SLITRK6

SLITRK6 Polyclonal Antibody for FC, IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, FC,ELISA

Conjugaison

Non conjugué

N° de cat : 21150-1-AP

Synonymes

SLIT and NTRK like protein 6, SLITRK6

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Galerie de données de validation

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  WB analysis of A549 using 21150-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21150-1-AP (SLITRK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21150-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21150-1-AP (SLITRK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 21150-1-AP

  Immunofluorescent analysis of HepG2 cells, using SLITRK6 antibody 21150-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 21150-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug SLITRK6 antibody (21150-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A549, tissu pulmonaire de souris
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21150-1-AP cible SLITRK6 dans les applications de WB, IF, FC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLITRK6 Protéine recombinante Ag15701
• Nom complet: SLIT and NTRK-like family, member 6
• Masse moléculaire calculée: 841 aa, 95 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: BC101070
• Symbole du gène: SLITRK6
• Identification du gène (NCBI): 84189
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLITRK6 is a member of the SLITRK family. Members of this family are integral membrane proteins that are characterized by two N-terminal leucine-rich repeat (LRR) domains and a C-terminal region that shares homology with trk neurotrophin receptors. This protein functions as a regulator of neurite outgrowth required for normal hearing and vision. Mutations in SLITRK6 gene are a cause of myopia and deafness.