Anticorps Polyclonal de lapin anti-SLMAP

• Phare
• Validé par KD/KO

SLMAP Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 25220-1-AP

Synonymes

KIAA1601, sarcolemma associated protein, Sarcolemmal associated protein, SLAP, SLMAP

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Galerie de données de validation

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  WB analysis of mouse heart using 25220-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25220-1-AP (SLMAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 25220-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25220-1-AP (SLMAP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 25220-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25220-1-AP (SLMAP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules A431, tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

25220-1-AP cible SLMAP dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLMAP Protéine recombinante Ag18162
• Nom complet: sarcolemma associated protein
• Masse moléculaire calculée: 828 aa, 95 kDa
• Poids moléculaire observé: 35 kDa, 45 kDa, 63-66 kDa, 83-91 kDa
• Numéro d’acquisition GenBank: BC115701
• Symbole du gène: SLMAP
• Identification du gène (NCBI): 7871
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sarcolemmal membrane associated protein (SLMAP) is a tail-anchored membrane protein involved in the docking and membrane fusion process via protein-protein interactions. Deregulation of SLMAP expression plays an important role in type 2 diabetes, and changes in SLMAP expression are linked to hyperglycemia and diabetes. SLMAPs are components of cardiac membranes and have been identified as a novel regulator of cardiac function. There are several isoforms of SLMAPs with varying molecular size (35, 45, 63, and 83-91 kDa) as a result of alternative splicing. The short 35-kDa isoform is predominantly expressed in cardiac and slow-twitch skeletal muscle, while the 83- to 91-kDa isoform is ubiquitous. This antibody recognizes all of these isoforms in mouse heart lysate. (21785580, 22180652)

Publications

Species	Application	Title
human	WB	Thorac Cancer; Tumor suppressor effect of an antibody on xenotransplanted sarcomatoid mesothelioma cells; Authors - Masayoshi Hasegawa ; KD Validated