Anticorps Recombinant de lapin anti-SMAD1

SMAD1 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242844B4

N° de cat : 85388-1-RR

Synonymes

242844B4, BSP1, BSP-1, hSMAD1, JV41

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Galerie de données de validation

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  WB analysis using 85388-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85388-1-RR (SMAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 85388-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 85388-1-RR (SMAD1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 85388-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMAD1 Recombinant antibody (85388-1-RR, Clone:242844B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 85388-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85388-1-RR against Human SMAD1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules HT-1080, cellules HUVEC, cellules RAW264.7
• Résultats positifs en IHC: tissu d'estomac de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85388-1-RR cible SMAD1 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SMAD1 Protéine recombinante Ag0701
• Nom complet: SMAD family member 1
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC001878
• Symbole du gène: SMAD1
• Identification du gène (NCBI): 4086
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase.

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