Anticorps Polyclonal de lapin anti-SMAD5

• Phare
• Validé par KD/KO

SMAD5 Polyclonal Antibody for FC, IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

20

N° de cat : 12167-1-AP

Synonymes

Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5

---

Galerie de données de validation

• 

  WB analysis of K-562 using 12167-1-AP

  WB result of SMAD5 antibody (12167-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD5 transfected K-562 cells.

• 

  WB analysis of K-562 using 12167-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12167-1-AP (SMAD5 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of K-562 using 12167-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12167-1-AP (SMAD5 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of K-562 using 12167-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12167-1-AP (SMAD5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 12167-1-AP

  IP Result of anti-SMAD5 (IP:12167-1-AP, 4ug; Detection:12167-1-AP 1:1000) with K-562 cells lysate 3200ug.

• 

  IF Staining of MCF-7 using 12167-1-AP

  Immunofluorescent analysis of MCF-7 cells, using SMAD5 antibody 12167-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of MCF-7 using 12167-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug SMAD5 antibody (12167-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Applications testées

• Résultats positifs en WB: cellules K-562,
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IF: cellules MCF-7
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 18 publications below
• FC: See 1 publications below

Informations sur le produit

12167-1-AP cible SMAD5 dans les applications de WB, IP, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMAD5 Protéine recombinante Ag2809
• Nom complet: SMAD family member 5
• Masse moléculaire calculée: 465 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC009682
• Symbole du gène: SMAD5
• Identification du gène (NCBI): 4090
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.

Publications

Species	Application	Title
		PLoS Biol; BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics.; Authors - Christian Hiepen
human	FC	Sci Rep; IRS4, a novel modulator of BMP/Smad and Akt signalling during early muscle differentiation.; Authors - Gina Dörpholz
rat	WB	Int J Biochem Cell Biol; The Role of SIRT6 in the Differentiation of Vascular Smooth Muscle Cells in Response to Cyclic Strain.; Authors - Qing-Ping Yao
mouse	WB	Sci Rep; Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.; Authors - Xueni Wang
mouse	WB	PLoS One; Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice.; Authors - Zhang Xing-Yi XY
human	WB	Biochem Biophys Res Commun; SPG6 supports development of acute myeloid leukemia by regulating BMPR2-Smad-Bcl-2/Bcl-xl signaling.; Authors - Jinliang Chen