Anticorps Polyclonal de lapin anti-SMARCA2-Specific

• Phare
• Validé par KD/KO

SMARCA2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 26613-1-AP

Synonymes

SMARCA2, EC:3.6.4.-, BRM, BRG1-associated factor 190B, BAF190B

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Galerie de données de validation

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  WB analysis of HeLa using 26613-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26613-1-AP

  WB result of SMARCA2-Specific antibody (26613-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA2-Specific transfected HeLa cells.

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  WB analysis of NIH/3T3 using 26613-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 26613-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 26613-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 26613-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26613-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 26613-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMARCA2-Specific antibody (26613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MDA-MB-453s, cellules NIH/3T3
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• CoIP: See 1 publications below

Informations sur le produit

26613-1-AP cible SMARCA2-Specific dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMARCA2-Specific Protéine recombinante Ag25157
• Nom complet: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
• Masse moléculaire calculée: 181 kDa
• Poids moléculaire observé: 180-190 kDa
• Numéro d’acquisition GenBank: NM_003070
• Symbole du gène: SMARCA2
• Identification du gène (NCBI): 6595
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Publications

Species	Application	Title
rat	WB	Neuron; Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination.; Authors - Zhixiong Liu
human	WB	Oncol Lett; PMID: 30127991; Authors - Xiaoli Xu
mouse	WB	Stem Cells; Chromatin Remodeling Factor BRG1 Regulates Stemness and Chemosensitivity of Glioma Initiating Cells.; Authors - Debolina Ganguly
mouse	WB	Biochem Biophys Res Commun; Chromatin remodeler BRM is a key mediator of leucine-stimulated mTOR gene transcription in mouse mammary epithelial cells; Authors - Changping Ke
human	CoIP	Nat Commun; Prion-like domain mediated phase separation of ARID1A promotes oncogenic potential of Ewing's sarcoma; Authors - Yong Ryoul Kim
human	WB	Cell Rep; SWI/SNF complex-mediated ZNF410 cooperative binding maintains chromatin accessibility and enhancer activity; Authors - Siyuan Xu

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