Anticorps Polyclonal de lapin anti-SMARCC1

SMARCC1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 17722-1-AP

Synonymes

BAF155, BRG1 associated factor 155, CRACC1, Rsc8, SMARCC1, SRG3, SWI3

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Galerie de données de validation

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  WB analysis using 17722-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17722-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17722-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 17722-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 17722-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17722-1-AP

  IP Result of anti-SMARCC1 (IP:17722-1-AP, 4ug; Detection:17722-1-AP 1:600) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules Jurkat, tissu cérébral de rat, tissu testiculaire de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral humain, tissu ovarien humain, tissu placentaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

17722-1-AP cible SMARCC1 dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMARCC1 Protéine recombinante Ag12109
• Nom complet: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
• Masse moléculaire calculée: 1105 aa, 123 kDa
• Poids moléculaire observé: 155-160 kDa
• Numéro d’acquisition GenBank: BC050564
• Symbole du gène: SMARCC1
• Identification du gène (NCBI): 6599
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMARCC1, also named as SWI/SNF complex 155 kDa subunit or BAF155, is a 1105 amino acid protein, which belongs to the SMARCC family. SMARCC1 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. SMARCC1 is Involved in transcriptional activation and repression of select genes by chromatin remodeling. SMARCC1 may stimulate the ATPase activity of the catalytic subunit of the complex. SMARCC1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calculated molecular weight of TCEA1 is 123 kDa, but modified SMARCC1 is about 155-160 kDa. (PMID: 24025402)

Publications

Species	Application	Title
human	WB,IP	Cancer Discov; A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction.; Authors - Jinxiu Li
mouse	CoIP	Nucleic Acids Res; HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.; Authors - Xu Zhu
		Mol Cell Biol; Growth Factor Independence 1B-Mediated Transcriptional Repression and Lineage Allocation Require Lysine-Specific Demethylase 1-Dependent Recruitment of the BHC Complex.; Authors - David McClellan