Anticorps Polyclonal de lapin anti-SMARCD3

SMARCD3 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12838-1-AP

Synonymes

BAF60C, BRG1 associated factor 60C, CRACD3, Rsc6p, SMARCD3

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Galerie de données de validation

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  WB analysis using 12838-1-AP

  C6 and NIH3T3 cells were subjected to SDS PAGE followed by western blot with 12838-1-AP (SMARCD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 12838-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12838-1-AP (SMARCD3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12838-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12838-1-AP (SMARCD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12838-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12838-1-AP (SMARCD3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12838-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12838-1-AP (SMARCD3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human heart using 12838-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human heart tissue using 12838-1-AP (SMARCD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human heart using 12838-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human heart tissue using 12838-1-AP (SMARCD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse pancreas using 12838-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 12838-1-AP (SMARCD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse pancreas using 12838-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 12838-1-AP (SMARCD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules C6, cellules BxPC-3, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cardiaque humain, tissu pancréatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

12838-1-AP cible SMARCD3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMARCD3 Protéine recombinante Ag3896
• Nom complet: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54-55 kDa
• Numéro d’acquisition GenBank: BC002628
• Symbole du gène: SMARCD3
• Identification du gène (NCBI): 6604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMARCD3 is a component of the SWI/SNF (mating type switching/sucrose non-fermenting) chromatin remodeling complex, which evolutionarily conserved from yeast to human, and all complexes contain a core set of conserved components, including BRG1/Brm-associated factors (BAFs) and a DNA-dependent SWI2/SNF2-like ATPase, which enables chromatin remodeling. SMARCD3 isoforms bind to several nuclear receptors and transcription factors of various families. SMARCD3 proteins interact in a ligand-independent manner with peroxisome proliferator- activated receptor gamma and enhance its transcriptional activity.

Publications

Species	Application	Title
human	WB	Cell Discov; m6Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.; Authors - Wei Zhuo
mouse	WB	Cardiovasc Res; The G protein coupled receptor Apj is expressed in the second heart field and regulates Cerberus-Baf60C axis in embryonic stem cell cardiomyogenesis.; Authors - D'Aniello Cristina C
human	WB	Transl Oncol; SNF5, a core subunit of SWI/SNF complex, regulates melanoma cancer cell growth, metastasis, and immune escape in response to matrix stiffness.; Authors - Ying Chen
human	WB	Cells; The SMARCD Family of SWI/SNF Accessory Proteins Is Involved in the Transcriptional Regulation of Androgen Receptor-Driven Genes and Plays a Role in Various Essential Processes of Prostate Cancer; Authors - Iris E Ertl