Anticorps Polyclonal de lapin anti-SMC1A

SMC1A Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 21695-1-AP

Synonymes

CDLS2, DXS423E, KIAA0178, SB1.8, SMC 1 alpha, SMC 1A, SMC protein 1A, SMC1, SMC1A, SMC1alpha, SMC1L1, SMCB

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Galerie de données de validation

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  WB analysis using 21695-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21695-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 21695-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21695-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 21695-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 21695-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21695-1-AP (SMC1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 21695-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21695-1-AP (SMC1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules K-562, cellules NIH/3T3
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

21695-1-AP cible SMC1A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMC1A Protéine recombinante Ag16286
• Nom complet: structural maintenance of chromosomes 1A
• Masse moléculaire calculée: 1233 aa, 143 kDa
• Poids moléculaire observé: 143-150 kDa
• Numéro d’acquisition GenBank: BC112127
• Symbole du gène: SMC1A
• Identification du gène (NCBI): 8243
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Structural maintenance of chromosomes protein 1A (SMC1A) is involved in chromosome cohesion during cell cycle and in DNA repair. It is a central component of cohesin complex which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Publications

Species	Application	Title
human	WB	Front Immunol; Interactome of the Autoimmune Risk Protein ANKRD55.; Authors - Nerea Ugidos
human	WB,IHC	Cancer Med; PIK3R3 is upregulated in liver cancer and activates Akt signaling to control cancer growth by regulation of CDKN1C and SMC1A; Authors - Weidong Lin
human	WB	Front Genet; In-Frame Variants in STAG3 Gene Cause Premature Ovarian Insufficiency.; Authors - Wen-Juan Xiao