Anticorps Polyclonal de lapin anti-SMCR7L/MID51

• Phare
• Validé par KD/KO

SMCR7L/MID51 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

63

N° de cat : 20164-1-AP

Synonymes

HSU79252, MID51, MIEF1, SMCR7L, SMCR7L/MID51

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Galerie de données de validation

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  WB analysis using 20164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20164-1-AP (SMCR7L/MID51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 20164-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20164-1-AP (SMCR7L/MID51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 20164-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 20164-1-AP (SMCR7L/MID51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20164-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20164-1-AP (SMCR7L/MID51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of RAW 264.7 using 20164-1-AP

  IP Result of anti-SMCR7L/MID51 (IP:20164-1-AP, 3ug; Detection:20164-1-AP 1:1000) with RAW 264.7 cells lysate 2400ug.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293T, cellules HT-1080, cellules NIH/3T3, RAW264.7, tissu testiculaire de souris
• Résultats positifs en IP: cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 62 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

20164-1-AP cible SMCR7L/MID51 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMCR7L/MID51 Protéine recombinante Ag13775
• Nom complet: Smith-Magenis syndrome chromosome region, candidate 7-like
• Masse moléculaire calculée: 463 aa, 51 kDa
• Poids moléculaire observé: 48-51 kDa
• Numéro d’acquisition GenBank: BC002587
• Symbole du gène: SMCR7L
• Identification du gène (NCBI): 54471
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human SMCR7L gene encodes, MID51, the mitochondrial dynamic protein of 51 kDa (also called mitochondrial elongation factor 1, MIEF1). MID51 is a single-pass membrane protein anchored to the mitochondrial outer membrane and regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Elevated MID51 levels induce extensive mitochondrial fusion, whereas depletion of MID51 causes mitochondrial fragmentation. MID51 interacts with and recruits Drp1 to mitochondria, suggesting a critical role of MID51 in regulation of mitochondrial fusion-fission machinery in vertebrates.

Publications

Species	Application	Title
human		Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KO Validated
human	WB	Autophagy; Loss of MIEF1/MiD51 confers susceptibility to BAX-mediated cell death and PINK1-PRKN-dependent mitophagy.; Authors - Hongxu Xian ; KD Validated
mouse	WB	Sci Adv; Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.; Authors - Xinwen Zhang
human	WB	Nat Commun; Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease.; Authors - Di Hu

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