Anticorps Polyclonal de lapin anti-SMIM1

SMIM1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 27849-1-AP

Synonymes

hypothetical LOC388588, LOC388588, Small integral membrane protein 1, SMIM1

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Galerie de données de validation

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  WB analysis of HEK-293 using 27849-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27849-1-AP (SMIM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 27849-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27849-1-AP (SMIM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 27849-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27849-1-AP (SMIM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu rénal de souris, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

27849-1-AP cible SMIM1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMIM1 Protéine recombinante Ag27028
• Nom complet: hypothetical LOC388588
• Poids moléculaire observé: 9 kDa
• Numéro d’acquisition GenBank: BC146957
• Symbole du gène: SMIM1
• Identification du gène (NCBI): 388588
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Small integral membrane protein 1(SMIM1) is a type II transmembrane phosphoprotein and displays the Vel blood group antigen at its carboxyl-terminus. SMIM1 is highly expressed in hematopoietic cells. It is associated with a variety of immune responses and plays an important role in RBC differentiation. SMIM1 can be used as a potential biomarker for the diagnosis of IDD (PMID: 30906890; 26452714).

Publications

Species	Application	Title
human	WB	Transfus Med Hemother; CRISPR/Cas9-Editing K562 Cell Line as a Potential Tool in Transfusion Applications: Knockout of Vel Antigen Gene; Authors - Jiaxuan Yang ; KO Validated

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