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Anticorps Monoclonal anti-SMN (Human,Mouse,Rat)

SMN (Human,Mouse,Rat) Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

3C10B5

N° de cat : 60154-2-Ig

Synonymes

C BCD541, Component of gems 1, Gemin 1, SMN, SMN (Human,Mouse,Rat), SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein

Galerie de données de validation

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  • Western Blot (WB) analysis of HeLa cells using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    WB analysis of HeLa using 60154-2-Ig

    WB result of SMN (Human,Mouse,Rat) antibody (60154-2-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN (Human,Mouse,Rat) transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    WB analysis using 60154-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    WB analysis of A375 using 60154-2-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    IHC staining of mouse liver using 60154-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    IHC staining of mouse liver using 60154-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    IHC staining of mouse brain using 60154-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    IHC staining of mouse brain using 60154-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    IF Staining of HepG2 using 60154-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN (Human,Mouse,Rat) antibody (60154-2-Ig, Clone: 3C10B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Applications testées

Résultats positifs en WBcellules HepG2, cellules A375, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules MOLT-4, cellules Raji, cellules T-47D
Résultats positifs en IHCtissu hépatique de souris, tissu cérébral de souris
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IFcellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochimie (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

60154-2-Ig cible SMN (Human,Mouse,Rat) dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène SMN (Human,Mouse,Rat) Protéine recombinante Ag14333
Nom complet survival of motor neuron 2, centromeric
Masse moléculaire calculée 282 aa, 30 kDa
Poids moléculaire observé 38 kDa
Numéro d’acquisition GenBankBC000908
Symbole du gène SMN2
Identification du gène (NCBI) 6607
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-2-Ig can recognize human,mouse and rat SMN gene.

Protocole

Product Specific Protocols
WB protocol for SMN (Human,Mouse,Rat) antibody 60154-2-IgDownload protocol
IHC protocol for SMN (Human,Mouse,Rat) antibody 60154-2-IgDownload protocol
IF protocol for SMN (Human,Mouse,Rat) antibody 60154-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols