Anticorps Polyclonal de lapin anti-SMNDC1

SMNDC1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 12178-1-AP

Synonymes

30 kDa splicing factor SMNrp, SMN related protein, SMNDC1, SMNR, SPF30

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Galerie de données de validation

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  WB analysis using 12178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 12178-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12178-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 12178-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12178-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12178-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 12178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12178-1-AP (SMNDC1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lymphoma using 12178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12178-1-AP (SMNDC1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lymphoma using 12178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12178-1-AP (SMNDC1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12178-1-AP (SMNDC1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu cardiaque humain, tissu de muscle squelettique de souris, tissu hépatique humain, tissu pulmonaire humain
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

12178-1-AP cible SMNDC1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMNDC1 Protéine recombinante Ag2822
• Nom complet: survival motor neuron domain containing 1
• Masse moléculaire calculée: 238 aa, 27 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC011234
• Symbole du gène: SMNDC1
• Identification du gène (NCBI): 10285
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Survival motor neuron domain-containing protein 1 (SMNDC1), also known as survival of motor neuron-related-splicing factor 30 (SPF30) or SMN-related protein (SMNR), is a constituent of the spliceosome complex that plays an essential role in spliceosome assembly by recruiting U4/U5/U6 tri-snRNP to the pre-spliceosomal complex (PMID: 9817934; 11331595; 30904945). Both SMNDC1 and SMN1 contain a highly conserved central Tudor domain which functions as a protein-protein interaction surface and mediates binding to the spliceosomal Sm proteins (PMID: 11331595).