Anticorps Recombinant de lapin anti-SMPD2

SMPD2 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240400B5

N° de cat : 83520-3-RR

Synonymes

N-SMase, Neutral sphingomyelinase, Lyso-platelet-activating factor-phospholipase C, Lyso-PAF-PLC, EC:3.1.4.12

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Galerie de données de validation

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  WB analysis using 83520-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83520-3-RR (SMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of U2OS using 83520-3-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMPD2 Recombinant antibody (83520-3-RR, Clone:240400B5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using 83520-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMPD2 Recombinant antibody (83520-3-RR, Clone:240400B5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83520-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83520-3-RR against Human SMPD2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293T, cellules MOLT-4, cellules SH-SY5Y
• Résultats positifs en FC (Intra): cellules HeLa, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83520-3-RR cible SMPD2 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SMPD2 Protéine recombinante Ag7367
• Nom complet: sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC000038
• Symbole du gène: SMPD2
• Identification du gène (NCBI): 6610
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMPD2, Sphingomyelin phosphodiesterase 2, is also named as Lyso-platelet-activating factor-phospholipase C and neutral sphingomyelinase (nSMase). It is involved in the sphingolipid metabolism pathway, which can catalyze the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Smpd2/Smpd3 double-mutant mice were found to have severe retardation of late embryonic and postnatal growth and delayed puberty, belonging to hypothalamus-induced combined pituitary hormone deficiency (PubMed: 15764706).

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